Standard Operating Procedure (SOP) for Dressing Cabinet in Sterile Area of Microbiological Lab
This SOP outlines the procedure for
using the dressing cabinet in the sterile area of the microbiological lab,
ensuring all personnel adhere to strict contamination control standards.
1. Objective
To provide a detailed procedure for
the proper use of the dressing cabinet in the sterile area of the
microbiological lab, ensuring maintenance of sterility and prevention of
contamination.
2. Responsibility
All personnel entering the sterile
area, including lab technicians, microbiologists, and other relevant staff, are
responsible for following this SOP.
3. Procedure
3.1. Preparation
Personal Hygiene:
Ensure hands and face are clean
before entering the dressing area.
Remove all jewelry, watches, and
other accessories.
Entry Protocol:
Enter the designated dressing area
through the main entrance.
Log your entry time in the lab
logbook.
3.2. Gowning Procedure
Gowning Materials:
Use only sterile gowns, gloves,
masks, caps, and shoe covers provided in the dressing cabinet.
Dressing Steps:
Step 1: Put on a hair cover,
ensuring all hair is completely enclosed.
Step 2: Put on a face mask, ensuring
it covers both nose and mouth securely.
Step 3: Wear a sterile gown,
ensuring it covers your body completely. Fasten all ties and snaps securely.
Step 4: Put on shoe covers, ensuring
they cover your shoes completely.
Step 5: Sanitize hands using the
provided hand sanitizer.
Step 6: Put on sterile gloves,
ensuring they cover the cuffs of the gown.
3.3. Exit Protocol
Removal of Gowning Materials:
Remove gloves and discard them in
the designated waste bin.
Remove gown carefully and discard it
in the designated waste bin.
Remove shoe covers, mask, and hair
cover, and discard them in the designated waste bin.
Sanitize hands after removing all
gowning materials.
Logging Out:
Log your exit time in the lab
logbook.
4. Cleaning and Maintenance
Daily Cleaning:
Ensure the dressing cabinet and
surrounding area are cleaned and sanitized daily.
Replenish all gowning materials as
needed.
Weekly Inspection:
Conduct a weekly inspection of the
dressing cabinet and gowning materials for any signs of contamination or
damage.
Replace any damaged or contaminated
items immediately.
5. Precautions
Sterility:
Always use sterile gowning
materials.
Ensure hands are sanitized before
and after gowning.
Contamination Control:
Do not touch any surfaces after
gowning to avoid contamination.
Report any breaches in sterility
immediately to the lab supervisor.
By following this SOP, all personnel
can help maintain a sterile environment in the microbiological lab, ensuring
the integrity of experiments and safety of lab workers.
END OF THE DOCUMENT
You may like to read these links:
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the Animal House Facility
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