SOP FOR WATERING AND FEEDING OF ANIMALS | STANDARD OPERATING PROCEDURE FOR WATERING AND FEEDING OF ANIMALS

                   1.0                            OBJECTIVE

1.1                            To lay down a standard procedure to be followed for watering and feeding of animals in Animal House Facility

2.0                            SCOPE

2.1                            This Standard Operating Procedure (SOP) shall be applicable to watering and feeding of animals in Animal House Facility.

3.0                            RESPONSIBILITY

3.1                            Attendant- Animal House Facility

3.2                            Head- Animal House Facility

4.0                            DEFINITIONS

4.1                            Nil

                   5.0                            PROCEDURE

5.1                            Designated/authorized staff shall make an entry into the small animal facility and exit from a small animal the facility as per the procedures described in the current version of SOP……….

5.2                            Watering of Animals

5.2.1                   Water bottles filled with fresh purified water shall be given to animals every day, or as and when necessary. Water passed through a water purifier shall be used for filling the water bottles. The filled water bottles shall be taken to the respective test/animal rooms on a trolley /tray. The used water bottles shall be replaced from the animal cages from left to right starting from the upper row first and water bottles shall be placed on the animal cages from left to right starting from the upper row.

5.2.2                    Water bottles shall be checked 2-3 times daily to ensure that the animals have access to fresh purified water all the time.

5.2.3                    In case of any water bottle/s found leaky /damaged / half-filled, it shall be replaced with a new water bottle.

5.2.4                   Water bottle and stoppers shall be washed once weekly or as and when required.

5.2.5                       Record of water offered to animals shall be documented in the format No. F...............

5.3                            Feeding

                    

5.3.1                    After receiving, feed shall be stored in Stainless steel (SS) container (for non-sterile feed). Feed shall be transferred to sterilized SS container after sterilization (as per SOP......).

5.3.2                    The designated/authorized staff of the respective room shall take the feed from the labeled sterilized feed storage bin bearing the batch number of feed-in use (if applicable), and the feed shall be transferred to a clean labeled Feed Offering Container and taken to the respective test/animal room for feeding the animals.

5.3.3                    The feed shall be filled up in each feed hopper using a feed scoop (spoon) working in a systematic manner from left to right starting from the upper row (Rodents) or from top to bottom of the cages. When all cages are filled, the feed container shall be kept in the designated area in the storage room. Optionally feed offered to individual cages shall be weighed as and when required or as per the study plan.

5.3.4                    The feed storage container and the food scoop shall be sanitized on a monthly basis. A record shall be maintained as per Format No.:-F/SOP/AHF/011/01-00.

5.3.5                    Feed shall be checked for the presence of moisture or contaminants before offering to animals.

5.3.6                    If a supplemental diet (i.e. lettuce, carrots, seeds, etc.) is indicated, same shall be placed in the proper amount in a site where an animal (s) has ready access to an item (s).

6.0                         ENCLOSURES

6.1                            Formats

Sr. No.	Format Title	Format No	No. of Pages
1.	Animal Feed and water record	F…………………..	01

7.0                            ABBREVIATIONS

7.1                            SOP                     : Standard Operating Procedure

7.2                            QAD                    : Quality Assurance Department

7.3                            AHF                     : Animal House Facility

8.0                            REFERENCES

8.1                        Committee for the purpose of control and supervision of experiments on animals (CPCSEA) guidelines for laboratory animal facility-2005, CPCSEA, Standard Operating Procedures for Institutional Animal Ethics Committee (IAEC), Animal Welfare Division, Ministry of Environment and Forests, January, 2010.

8.2                        Guide for the care & use of laboratory animals, 8th Edition, National Research Council, National Academy Press, Washington, D.C. 2010.

9.0                            REVISION HISTORY

Sr. No.	Change Control No.	Reason for change

                                                                                                                 

END OF DOCUMENT

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