1.0 PURPOSE
To design the standard operating procedure for operation of Ultrasonic Probe Sonicator.
2.0 SCOPE
2.1 This SOP shall be applicable for the operation of Ultrasonic Probe Sonicator.
3.0 RESPONSIBILITY
3.1 All the users including Scientist(s) and Research associate(s) Invitro Laboratory are responsible for implementation of this SOP and maintaining the Ultrasonic Probe Sonicator.
4.0 DISTRIBUTION
4.1 The Quality Assurance department responsible to keep SOP ‘Master Copy’ approved through Quality Manager.
4.2 The copy of ‘Control Copy’ of this SOP is being distributed in invitro lab and placed near related Equipment/ Instrument as ‘Display Copy’.
5.0 ABBREVIATIONS and DEFINITIONS
5.1 Abbreviations
5.1.1 SOP : Standard Operating Procedure
5.2 Definitions
5.2.1 Ultrasonic Probe Sonicator is a instrument apply sound energy to agitate particles in a sample such as the extraction of multiple compounds from plants/animals. Ultrasonic frequencies (>20 kHz) are usually used, leading to the process also being known as ultrasonication.
6.0 PROCEDURE
6.1 Operation
6.1.1 Turn the instrument on by pressing the ON/OFF switch located on the rear panel near the AC power input. A Power LED on the front of the panel will glow to indicate the instrument is on.
6.1.2 The control panel consists of 3 knobs and a “RUN” and “STOP” button. Turning the control knobs to the right increases the levels of these settings.
6.1.3 The timer LED will light when the timer knob is set to the desired time. The timer is adjustable from 1 to 15 minutes.
6.1.4 The pulser knob allows for two different power output modes - constant mode & pulse mode. The pulse duration can be adjusted from 10% to 90%, enabling a solution to be processed at full power range while limiting temperature. The pulse mode is especially valuable for processing heat sensitive samples. The constant mode is operative when the knob is in the fully counter clockwise position.
6.1.5 Immerse the tip in the appropriate solution. Tip depth should be approximately equal to 1.5 times the diameter of the tip. Press the “Start” button to deliver the ultrasonic power to the convertor. If the Timer is used, the processing will automatically stop when the Timer is through. Push the “Reset” button to terminate the processing at any time.
6.1.6 Switch off the instrument after use.
6.2 Cleaning
6.2.1 Always disconnect the power cord before cleaning
6.2.2 The housing of the unit can be cleaned with a sponge or a damp cloth moistened with water, alcohol or an acid-free cleaning solution.
6.2.3 Titanium Processing Tips can be cleaned with isopropyl alcohol or sterilized in an autoclave.
6.2.4 Do not spray water or alcohol directly on the unit, especially on the front control panel, in the air vents, and the fan.
6.2.5 In case of a spill, decontaminate parts that may have been contaminated with an appropriate disinfectant. The decontamination procedure is the sole responsibility of the user. Parts that may have been contaminated can be cleaned with a sponge or a damp cloth moistened with a non-abrasive cleanser. In case another decontamination procedure is to be applied, please first contact technical support to ensure the compatibility of the new procedure with the instrument.
7.0 PRECAUTIONS
7.1 Do not touch the titanium processing tip while power is being delivered to the tip.
7.2 Ultrasonic Homogenizers use sound waves to agitate mixtures. This can cause rapid heating and localized points of increased pressure. They should not be used with sealed flasks or with flammable liquids, especially low-boiling solvents (such as ether).
7.3 When a liquid is subjected to ultrasonic energy at or above the threshold of cavitation, audible sounds are emitted from the vessel containing the liquid. These sounds can be irritating to the operator and care should be taken to avoid prolonged exposure.
7.4 The instrument should not be operated at its maximum power level for more than fifteen (15) minutes. Long periods of operation will shorten the life of the electronic components due to the high temperature developed inside the unit.
8.0 REFERENCE(S) AND FORMAT(S)
8.1 Reference
8.1.1 User manual.
8.2 Format
8.2.1 Format I- Document History sheet.
8.2.1 Format II- Format for Log Book.
9.1 Maintain the history of SOP as per laboratory format.
END OF THE DOCUMENT
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