Study Points in Male Pattern Baldness Model for Pre-clinical Studies

 1. How Finasteride can be used as a standard drug in Male Pattern Baldness Studies of mice?

Ans:- Here Finasteride blocks the action of 5-alpha reductase and precludes the conversion of testosterone in Dihydrotesterone (DHT). Dihydrotestosterone mainly damages the hair follicles and synergizes the effect of baldness. 

2. How does Dihydrotestosterone causes Benign Prostate Hyperplasia BPH? 

Ans:- DHT is mainly due to unknown reasons, it increases the production of the epithelial and stromal cells in the periurethral area. This phenomenon causes pressure on the urethra and urinary bladder, produces a detrimental effect on the bladder, and gradually bladder loses its capacity for complete emptying, thus it bases on urgency, obstruction, and more frequent urination in BPH.

Treatment: Manly doctors prescribe Finasteride to that reduce the production of DHT by inhibiting 5 alpha reductase (5aR) enzymes. 5 alpha reductase has 3 three isozymes of 5 alpha-reductase encoded by the genes SRD5A1, SRD5A2, and SRD5A3. Finasteride inhibits only two isozymes SRD5A2, and SRD5A3. Whereas another finasteride agonist Dutasteride reduced all three forms of isozymes in the prostate. Another alpha reductase such as Prozocin also has a good role in DHT level reduction, more adversely it causes low blood pressure, due to the dilation of all blood vessels. And due to low blood pressure often old adults feels sudden dizziness and fall. Thus the case of bone fracture is more in elderly persons due to Prozocin. Tamsulosin is more specific than Dautsteride and Prazocin inhibits SRD5A1, amends the effect more profoundly, and gives more patient satisfaction.

3. Does the Bicalutamide used in Male Pattern Baldness?

Ans: Bicalutamide has an antiandrogen effect thus it’s mainly used the inhibit the spread of prostate cancer and has more side than Finasteride, Dautsteride, and Prazosin. Yes, in combination with DHT, it can be used to induce male Pattern baldness in mice

4. What causes DHT in mice?

Ans: DHT was found to bring early hair regression, miniaturization, density loss, and changes in hair morphology in male C57BL/6 mice.

5. How to give DHT in mice and what will be the vehicle, in which DHT can be easily dissolved?

Ans: DHT is administered intraperitoneal by dissolving in corn oil at the dose of 2mg/d.

6. What will be the Normal Range of DHT in Humans and Mice:

Ans:  In humans, the DHT range is around 250-990 pg/ml, as per the Lal path lab report format, and in mice, the DHT level is around 120 pg/ml, in the literature survey.

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