STP FOR FOR DETERMINATION OF REFRACTIVE INDEX IN OIL AND FAT

1.0 PURPOSE

The purpose of the document is to establish a general and uniform method for the refractive index in oil and fat.

2.0 SCOPE

This standard testing procedure is suitable for the determination of the refractive index in oil and fat.

3.0 RESPONSIBILITY

The expert and trained chemist are responsible for following this procedure. The technical manager is responsible for implementing this procedure.

4.0 REQUIREMENTS

4.1 Apparatus

4.1.1 Butyrometer /Refractometer

4.2 Glassware

NA

4.3 Reagent

NA

5.0 PROCEDURE

5.1 Wipe and clean the surface

5.2 Drip sample 100μl in the prism.

5.3 Press the start/off switch.

5.4 The refractive index is displayed in numerical characters.

5.5 Repress the start /off switch, so that the refractive index of the sample will be shown on the display blinks three times.

5.6 Note down the reading.

5.7 To turn off the display keep the start/off switch press for about 2 seconds.

6.0 CALCULATION

Temperature correction: Unless the correction factors are specified in the detailed specification.

Approximate correction shall be made using the following equation.

R=R’ +K (T’-T)

Where,

R= the reading of the Butyrometer/Refractometer reduced to the specified temp. (T°C)

R’= the reading at T’°C

K= 0.55 for fat &0.58 for oils

T’=the temp. at which the reading R is taken

T= the specified temp. (Generally 40°C)

7.0 REVISION/CHANGE HISTORY

8.0 ATTACHMENTS

· Appendix-1: FLOW CHART FOR REFRACTIVE INDEX

9.0 REFERENCES

· Clause no. 10 of IS 548 Part I:1964; (RA 2015), Amd.-5,1996

END OF THE DOCUMENT

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