STANDARD OPERATING PROCEDURE COLLECTION OF SERUM FROM WHOLE BLOOD

1.0 Purpose

This document describes the process for the collection of human serum from whole blood.

2.0 Scope

These guidelines apply to personnel intending to preserve serum biobanking studies

3.0 Requirements:

3.1. Equipment:

3.1.1 Centrifuge with swinging bucket rotor

3.1.2 -80°C Freezer

3.1.3 Biosafety Cabinet Hood

3.1.4 Pipette Aid

3.2. Materials:

3.2.1 Cryovials 

3.2.2 15ml and 50ml Sterile, Polypropylene, Conical, Centrifuge Tubes

3.2.3 Sterile pipets

3.2.4 Sterile cryovials

4.0 Method:

4.1 Filled red top blood collection tubes should sit upright after the blood is drawn at room temperature for a minimum of 30 to a maximum of 60 minutes to allow the clot to form. (Note: The red top tubes do not need to be full.) These tubes, without additives, allow the red blood cells to form a clot that includes white blood cells, platelets, etc.

4.2 Centrifuge the blood sample at the end of the clotting time (30-60 minutes) in a horizontal rotor for 20 minutes at 1300g at room temperature. If the blood is not centrifuged immediately after the clotting time, the tubes should be refrigerated for no longer than 4 hours.

4.3 Use the pipette to transfer the serum into labeled cryovials. Aliquot 100-250µl serum into cryovials. This process should be completed within 1 hour of centrifugation. (Note: Be very careful not to pick up red blood cells when aliquoting. This can be done by keeping the pipet above the red blood cell layer and leaving a small amount of serum in the tube).

4.4 Place all aliquots upright in a specimen box or rack in -80°C or colder freezer. All specimens should remain at -80°C or colder.

5.0 Record

1. Hemolysis of the serum occurred

2. Date and time of blood collection

3. Number and volume of aliquots prepared

4. Date and time into -80°C

5. Date and time of shipping

6. Any freeze-thaw that occurs with a sample for any reason

7. Any variations or deviations from the SOP, problems, or issues

                                                                       END OF THE DOCUMENT

You may like to read these links: 

1. List of All SOPs and Documents for the Microbiology Laboratory

2. List of All SOPs and Documents for In-vitro Laboratory

3. List of All SOPs and Documents for the In-vivo Laboratory.

4. List of All SOPs and Documents for Clinical Research

5. List of Instruments and Equipment Used In Laboratory Animal Facility

6. Animal  Facility Design - Small Laboratory Animals (Rat, Mice, Rabbit, Guinea Pig)

7. List of Chemicals Needed for In-vivo Laboratory

8. List of Physiological Data for Small Laboratory Animals 

9. List of All SOPs and Documents for the Animal House Facility

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