SOP for Animal Necropsy and Post-Mortem Examination: Procedure, Steps, and Differences Between Autopsy, Necropsy, and Post-Mortem
1.0
OBJECTIVE: To design a Standard Operating Procedure that describes the
procedure for animal Necropsy and Post-Mortem.
2.0
RESPONSIBILITY: It is the responsibility of Principal Investigator (PI), Animal
house In-charge and Animals Care Personals.
3.0
REQUIREMENTS:
3.1
Carbon Dioxide asphyxiation assembly
3.2
Pentobarbitone/Ketamine
3.3
Scissors
3.4
Scalpels/blades
3.5
Forceps
3.6
Personal Protection Equipment (PPE)
3.7
For tissue collection
3.7.1
10% neutral buffered formalin
3.7.2
Fixative of choice unless otherwise specified by study director
3.7.3
Volume should be 10 times the volume of immersed tissue
3.7.4
Leak proof wet tissue container
3.7.5
Size compatible with 10:1 formalin/tissue ratio
3.7.6
If blood sampling is required, blood should be collected as follows:
3.7.6.1
Purple top tube (EDTA)- whole blood/plasma
3.7.6.2
Red/Grey top tube or serum separator - serum
3.7.6.3
Red top tube (plain) or centrifuge tube – serum
3.7.6.4
Syringe- determined by amount needed
3.7.6.5
Micro capillary tubes
3.8
Labels
3.8.1
Animal identification number
3.8.2
Study number
3.8.3
Date
3.9
Necropsy form- description should be
3.9.1
Accurate and brief
3.9.2
Tissue required should be accounted for
3.9.3
Appropriate entries for
3.9.4
Tissue examination, saved and/or fixed
3.9.5
Assurance of animal identification
4.0 PROCEDURE:
4.1
Animal necropsy should be performed in areas specially designated for that
purpose.
4.2
Stainless steel tray having wax was used for necropsy and was de-contaminated
after each use.
4.3
Necropsy was done in a manner that avoids the occurrence of artifacts and
change in the tissue collection.
4.4
Animals that were not to be necropsied were immediately identified and
refrigerated and the on-duty Research staff was notified.
4.5
Proper personal protective equipment and attire was worn when performing
necropsy.
4.5.1
Disposable gloves, shoe cover and gown were worn when necropsy was being
conducted.
4.5.2
Full protective clothing was worn when the animals to be necropsied were infected
with bio hazardous materials or chemical carcinogens (double gloves, cap,
disposable gown, shoe covers, mask and eye protection).
4.6
Specimens for microbiological assessment were collected using sterile
instruments and aseptic technique.
4.7
All specimens, whether collected for submission to a laboratory for analysis or
for archival purposes were placed in a suitable container and labelled with Principal
Investigator, animal ID and date of collection. Besides this the tissue or
sample collected are appropriate and the fixative or specific storage
requirements were mentioned if found necessary.
4.8
All tissue submitted for histopathological assessment by a pathologist for
studies had all the necessary information to completely identify the specimen,
including the PI, animal ID, date of collection, tissue submitted, fixative
used or specific storage requirements, any special laboratory test or
procedures requested and the name of person conducting the necropsy.
5.0
PRECAUTIONS:
5.1
Carefully label the samples (blood or tissue).
5.2
All the details should be thoroughly checked by the PI and then the Quality
Assurance Personal.
5.3
All the equipment's used for necropsy should be washed, cleaned and dried
before the start of the necropsy.
5.4
Paraffin wax trays should be cleaned thoroughly to remove the blood stains and
other things and should be ready for reuse.
6.0
DEFINATIONS:
6.1
Autopsy: A specialized surgical and visual examination of a deceased human body
to determine the cause, manner, and mechanism of death. The word comes from
Greek roots meaning "to see with one's own eyes".
6.2
Necropsy: A post-mortem examination performed on a non-human animal (a carcass)
to figure out why the animal died or to investigate animal diseases.
6.3
Post-mortem: A broad term meaning literally "after death" (post =
after, mortem = death). It encompasses any evaluation, test, report, or
investigative procedure conducted after death—which includes autopsies,
toxicological tests, and external inspections.
7.0
FORMATS:
7.1
NECROPSY REPORT – FORMAT NO: SOP/LAF/046-F01
7.2
POST-MORTEM REPORT – FORMAT NO: SOP/LAF/046-F02
7.3 Animal - Post Mortem Report Format
7.4 Animal - Necropsy Report Format
8.0
REFERENCES:
8.1
Feldman, D.B. and Seely J.C., Necropsy Guide: Rodents and the rabbit. CRC Press
Inc., Boca Raton FI, 1998.
8.2
Pathology of domestic animals’ 3rd ed., vols. 1-3 Academic Press Inc., 1985.
END
OF THE DOCUMENT
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