STANDARD OPERATING PROCEDURE FOR NECROPSY AND POST MORTEM EXAMINATION

1.0 PURPOSE:

1.1 To outline the proper, complete, and consistent procedures for performing a necropsy and postmortem.

2.0 RESPONSIBILITY:

2.1 This SOP is intended for the use by Principle Investigator, Animal house in charge, and Animal care facility personnel.

3.0 DEFINITIONS

3.1. Prosector: the individual who performs the necropsy.

3.2. Scribe/assistant- The individual or individual who assists the prosector by verifying.

 3.2.1 Identify individual rodents

3.2.2. Accuracy and completeness of necropsy forms

3.2.3. Recording observation, photographs will be taken at the discretion of the attending veterinarian or with any unusual finding.

4.0 REQUIREMENTS

4.1. Materials required

4.1.1 CO2 asphyxiation assembly

4.1.2 Pentobarbitone/Ketamine

4.1.3 Scissors

4.1.4 Scalpels/blades

4.1.5. Forceps

4.2. Tissue collection

4.2.1. 10% neutral buffered formalin

4.2.2.  Fixative of choice unless otherwise specified by the study director

4.2.3. Volume should be 10 times the volume of immersed tissue

4.2.4. Leakproof wet tissue container

4.2.5. Size compatible with 10:1 formalin/tissue ratio

4.2.6. If blood sampling is required, blood should be collected as following instruction

4.2.6.1. Purple top tube (EDTA)- whole blood/plasma

4.2.6.2. Red/Grey top tube or microtiter (serum separator)- serum

4.2.6.3. Red top tube (plain) or centrifuge tube – serum

4.2.6.4. Syringe- determined by the amount needed

4.2.6.5. Micro capillary tubes

4.3. Labels

4.3.1. Animal identification number

4.3.2. Study number

4.3.3. Date

4.4.4. Necropsy form – description should be

4.4.5.Accurate and brief

4.4.6. Tissue required should be accounted for

4.4.7. Appropriate entries for

4.4.8 Tissue examination, saved and/or fixed

4.4.9.Assurance of animal identification

5.0 PROCEDURE

5.1. Animal necropsy should be performed in areas specially designated for that purpose.

5.2. Stainless steel tray having wax should be used for necropsy and decontaminated after each use.

5.3. Necropsy should be used in a manner that avoids the occurrence of artifacts or postmortem change in the tissue collection and soon after death as possible to ensure tissue integrity.

5.4. Animals that cannot be necropsied immediately should be identified and refrigerated and the attending research staff notified.

5.5. Proper personal protective equipment and attire must be worn when performing a necropsy.

5.5.1. Disposable gloves, shoe cover, and gown must be worn when conducting a necropsy.

5.5.2 Full protective clothing must be worn when handling animals infected with biohazardous materials or chemical carcinogens and includes double gloves, cap, disposable gown, shoe covers, mask, and eye protection.

5.5.3. Specimens for microbiological assessment must be collected using sterile instruments and aseptic techniques.

5.5.4. All specimens, whether collected for submission to a laboratory for analysis or for archival purposes must be placed in a suitable container and labeled as to the PI, animal ID, date of collection, the tissue or sample collected appropriate, and the fixative or specific storage requirements when necessary.

5.5.5. All tissue submitted for histopathological assessment by a pathologist for studies should have the necessary information to completely identify the specimen, including the PI, animal ID, date of collection, tissue submitted, a fixative used or specific storage requirements, any special laboratory test or procedures requested and the name of the person conducting the necropsy.

6.0. REFERENCES:

6.1. Feldman, D.B. and Seely J.C., Necropsy Guide: Rodents and the rabbit. CRC Press Inc., Boca Raton FI, 1998.

6.2. Pathology of domestic animals 3rd ed., vols. 1-3 Academic Press Inc., 1985.

END OF THE DOCUMENT

You may like to read these links: 

1. List of In-vivo laboratory Vendors (Capex)

2. List of SOPs and Documents for CPCSEA

3. List of All SOPs and Documents for Laboratory Instruments and Equipment

4. List of All SOPs and Documents for Animal House Facility

5. List of All SOPs and Documents for In-vivo Laboratory

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