What are the points need to be considered before starting a diabetic wound healing study on rabbits? | Standardization of Diabetes Model in Rabbits

 Standardization of Diabetes Model in Rabbits before Wound Generation

The following points need to be considered before starting a diabetic wound study on rabbits:

 1. Rabbits' weight should be more than 2 kg. 

 2. Provisions for the rabbit run should be arranged. rabbits are kept in rabbit run after diabetes induction. 

 3. Rabbits' food should not be given in the rabbit run.

 4. A single dose of Alloxan monohydrate (150 mg/kg) is needed intravenous infusion route to induce diabetes to New Zealand Rabbits.  

5. During diabetes induction with Alloxan there maybe chances of 5 types of variants may present in the diabetes study.

         a. Hypoglycemic 

         b. Hyperglycemic

         c. Insulin-induced (I) Hypoglycemic (II) Appetite loss

         d. Not Induced 

         e. Reversed to Non-diabetic

 6. Rabbits show a lower Blood Glucose Level (BGL) in the morning and high in the evening & you will find the highest insulin level every third day evening. Initially, since diabetes induction and up to diabetes stabilization in rabbits, it is necessary to take BGL twice a day.

7. 20- 25% animals remain not induced after the first dose of alloxan Injection; need to be re-induced after 48 hours.

8. Animals should be observe up to the first 48 after alloxan induction, there may be chances of hyperglycemic or hypoglycemic shock after the first 6 hrs.

9. Possibility of hypoglycemia is more in rabbits after the first 6 hours of alloxan induction. So it is necessary to take blood glucose level before 15 minutes of 6 hours.

10. In a diabetes study, more than 30 % of animals get die during the induction of diabetes. 

11. Rabbits diabetes study takes at least one month to stabilize the blood glucose level of more than 300 mg/dl.

12. 0.2 % Nitrofurazone is the best standard drug for diabetic wounds in rabbits.

13. Some parameters like wound area, % relatively wound area contraction, blood glucose level (BGL), Insulin consumption, epithelialization time, inflammatory phase,  proliferation phase, the single dose OGTT, Multiple-dose OGTT by AUC calculation are considered normal during this study.

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1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agent, Standard or Reference Drug and related parameters.

4.List of OECD Guidelines or Toxicological Studies

5.List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

6.EVALUATION OF PROTECTIVE EFFECT OF TEST SAMPLE IN SURGICALLY INDUCED NEUROPATHY PAIN USING CHRONIC CONSTRUCTIVE INJURY (CCI) MODEL IN EXPERIMENTAL RAT

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9.What are the points need to be considered before starting a diabetic wound healing study on rabbits?

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