STANDARD OPERATING PROCEDURE FOR SWAB ANALYSIS

1.0 OBJECTIVE

To lay down the procedure for collection of swab samples from surfaces of equipment and floor.

2.0 SCOPE

This SOP is applicable to the Animal House Facility and Microbiology Laboratory .

3.0 RESPONSIBILITY

In-charge Animal house shall collect the swab sample and will maintain the record.

In-charge Microbiology is responsible for overall compliance of this procedure.

4.0 PROCEDURE:

Collection of swab sample for microbiological analysis:

• Take about 2g of absorbent cotton and wrap it around one edge of a glass rod.

• Transfer this swab into a graduated, stoppered test tube, containing 5 ml of distilled water.

• Sterilize by autoclaving at 15 lbs pressure ﴾121°C﴿ for 15 minutes.

• Take the sterilized swab to the sampling area.

• Take out the swab from the test tube and swab the surface of the equipment/floor, covering an area of 10 x 10  sq. cm., in unidirectional movements and not to and for movements.

• After swabbing, place the swab in another sterile test tube containing 10 ml of sterile buffered peptone water.

• Shake the tube gently and let it stand for 15 minutes.

•  Remove the swab from the test tube and analyze the peptone water as per the specifications.

• Collection of swab sample for chemical analysis :

• Take about 2 g of absorbent cotton and wrap it around one edge of a glass rod.

• Transfer this swab into a clean, graduated, stopper test tube, containing 5 ml of distilled water.

• Take the swab to the sampling area.

• Take out the swab from the test tube and swab the surface of the equipment, covering an area of 10 x 10 sq. cm., in unidirectional movements and not to and for movements.

• After swabbing, place the swab in another test tube containing 10 ml Acetate Buffer pH 4.0.

•  Sonicate the contents of the tube for 15 minutes

• Transfer the contents to a 100 ml volumetric flask and make up the volume to 100 ml with Acetate Buffer pH 4.0.

• Analyze this solution as per the specifications.

5.0 PRECAUTIONS:

Protective laboratory coats, gowns, smocks, gloves or uniforms designated for lab use will be worn while in the laboratory.

All infectious agent cultures, stocks, animal carcasses and/or tissues, sharps used to inoculate animals, etc. and bedding from infected animals will be disposed of as hazardous waste (i.e. put in an appropriately labeled red biohazard bag, and decontaminated By autoclaving.


6.0 REFERENCES:
API, Part-I, Vol.-IX (Extracts); Appendices-3; pp 124

7.0 ABBREVIATIONS:

SOP : Standard operating procedure
QA : Quality assurance
QC : Quality control

API: Active Pharmaceutical Ingredient

  

                                                             END OF THE DOCUMENTS

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You may also like to see:

1. SOP for Microbial Analysis of Water

2. SOP for Specific Pathogens Salmonella or E.coli in Animal Urine

3.List of All SOPs and Documents for Animal House Facility

4. List of All SOPs and Documents for Microbiology Laboratory

5. List of All SOPs and Documents for In-vitro Laboratory

6. List of All SOPs and Documents for In-vivo Laboratory.

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