EVALUATION OF ANTUROLITHIASIS POTENTIAL OF PLANT EXTRACT(S) IN ETHYLENE GLYCOL INDUCED UROLITHIASIS WISTAR RATS

EVALUATION OF ANTUROLITHIASIS POTENTIAL OF PLANT EXTRACT(S) IN ETHYLENE GLYCOL INDUCED UROLITHIASIS WISTAR RATS

1.0  INTRODUCTION:

Urolithiasis is a nephrological disorder that denotes stones (Calcium oxalate, uric acid, struvite & cystine) originating anywhere in the urinary tract, including the kidneys and bladder, however, renal epithelial cells produce ROS (Reactive oxygen species) when exposed to different stones. Various therapies including thiazide diuretics and alkali-citrate are being used in an attempt to prevent the recurrence of hypercalciuria and hyperoxaluria-induced calculi.

 

2.0  TEST SYSTEM DETAILS:

Species             : Rattus norvegicus (Rat)

Strain                : Wistar

Age                   : 8-10 weeks

Body Wight        : 180-200 g

Sex                   : Male

No. of animals    : 8 /Group

3.0  ALLOCATION OF GROUPS:

Groups	Treatment	Dose; ROA (p.o.)	No. of Animals
G1	Normal Control	Normal saline or 0.25% Na-CMC	8
G2	Disease Control	Normal saline or 0.25% Na-CMC	8
G3	Reference Drug- Cystone	750 mpk; p.o.	8
G4	Test Compound-1	X mpk	8
G5	Test Compound -2	XX mpk	8

# 10-20% animals will be taken extra due to variability in the diabetes development and possibilities of animal mortalities 

4.0 METHODOLOGY:

4.1 Induction of urolithiasis

· Urolithiasis or hyperoxaluria will be induced in Wistar rats by feeding them with 0.75% w/v of ethylene glycol in drinking water for 28 consecutive days.

· Group G1 and G2 animals will be treated as normal control (without urolithiasis) and disease control (with urolithiasis).

· Both groups will be administered with normal saline or 0.25% Na- CMC.

· Animals of group G3 will be treated as reference control and administered Cystone at the dose of 750 mg/kg; p.o.

· Similarly, animals of groups G4 and G5 will be administered with test compounds at different doses.

· All the treatments will be started at 15 days after ethylene glycol and continued for 28 days.

4.2 Assessment of Antiurolithiatic Activity

All the animals will be kept in individual metabolic cages and urine samples of 24 h will be collected on the 28th day.

· All animals had free access to drinking water during the urine collection period and stored at 4°C.

· Urine will be analyzed for pH, urine volume, calcium, phosphate, and oxalate content.

· Furthermore, blood samples will be collected on day 28, and serum will be separated by centrifugation at 10 000 × g for 10 min.

· Serum samples will be processed for creatinine, uric acid and blood urea nitrogen (BUN) using the biochemical analyzer.

5.0 END POINT PARAMETER(S):

·       Body Weight

·       Renal Function Profile- Urea, Creatinine, Uric acid and BUN

·       Urine volume

·       Urine pH

 

6.0  REFERENCE(S):

6.1 Elias Edwin Jarald, Pankaj Kushwah, Sheeja Edwin, Suhail Asghar, Showkat Ahmad Patni. Effect of Unex on ethylene glycol-induced urolithiasis in rats. Indian Journal of Pharmacology August 2011 | Vol 43 | Issue 4.

6.2 Vaibhavkumar B. Patel, Niyati Acharya. Effect of Macrotyloma uniflorum in ethylene glycol induced urolithiasis in rats. Heliyon 6 (2020) e04253

 

6.3 Atul Makasanaa, Vishavas Ranpariya, Dishant Desai, Jaymin Mendpara, Vivek Parekh. Evaluation for the anti-urolithiatic activity of Launaeaprocumbens against ethylene glycol-induced renal calculi inrats. Toxicology Reports 1 (2014) 46–52

 

6.4 Narendra Vyas and Ameeta Argal. Antiurolithiatic Activity of Extract and Oleanolic Acid Isolated from the Roots of Lantana camara on Zinc Disc Implantation Induced Urolithiasis. Hindawi. Volume 2013, Article ID 951795, 5 pages http://dx.doi.org/10.1155/2013/951795

 

6.5 M.Nishanthi , B.Vijayakumar and M. Vijey Aanandhi. Antiurolithiatic activity of the plant extracts of Peperomia tetraphylla on ethylene glycol induced urolithiasis in rats. Rasayan journal of chemistry. Vol. 9 | No. 2 |294 - 299 | April - June | 2016

 

6.6 Bahuguna Y, Rawat MM, Juyal V, Gupta V. Antilithiatic effect of flower Jasmin auriculatum Vahl. Int J Green Pharm 2009; 3:155-63.

                                END OF DOCUMENT

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