1.0 INTRODUCTION:
Inflammation is a body response to injury, infection, or destruction
characterized by heat, redness, pain, swelling, and disturbed physiological
functions, caused by physical trauma, noxious chemical, or microbial agents. It
is the body's response to inactivate or destroy the invading organisms, remove
the irritants, and set the stage for tissue repair. It is triggered by the
release of chemical mediators from injured tissue and migrating cells. The
migration of leukocytes from the venous systems to the site of damage, and the
release of cytokines, are known to play a crucial role in the inflammatory
response. These chemicals cause the widening of blood capillaries (vasodilation)
and the permeability of the capillaries. This will lead to increased blood flow
to the injured site.
2.0 METHODOLOGY:
·
Egg Albumin Denaturation Method
·
The reaction mixture (5 ml)
will have consisted of 0.2 ml of fresh egg albumin, 2.8 ml of buffered phosphate
saline (PBS, pH 6.4), and 2.0 ml of different concentrations of Test samples (10,
30, and 100μg/ml).
·
The positive control will consist of 0.2 ml of fresh egg albumin, 2.8 ml of PBS (pH 6.4), and 2.0 ml of
diclofenac sodium at different concentrations (10, 30, and 100μg/ml).
·
Negative control samples will
be contained the same amount of egg albumin and PBS with 2.0 ml of distilled
water.
·
The mixture will be incubated
at 37± 2°C for 15 min and then heated at 70°C for 5 min to induce
denaturation.
·
After cooling, the absorbance
will be recorded at 660 nm using the vehicle as a blank.
·
The experiment will be carried
out in triplicates. The percentage inhibition of protein denaturation will be
calculated using the formula
(%)
Percentage inhibition = Ac – At/Ac × 100
Where
At is the absorbance of the test and Ac is the absorbance
of the control.
3.0 END POINT PARAMETER(S):
·
% Percentage inhibition activity
4.0 REFERENCE(S):
4.1 Douglas Bosco Aidoo, Daniels Konja, Isaac
Tabiri Henneh, and Martins Ekor. Protective Effect of Bergapten against Human
Erythrocyte Hemolysis and Protein Denaturation In Vitro. International Journal
of Inflammation Volume 2021, Article ID 1279359, 7 pages https://doi.org/10.1155/2021/1279359.
4.2 Yesmin, S., Paul, A., Naz, T. et
al. Membrane stabilization as a mechanism of the anti-inflammatory
activity of ethanolic root extract of Choi (Piper chaba). Clin
Phytosci 6, 59 (2020). https://doi.org/10.1186/s40816-020-00207-7
4.3 K. D. P. P. Gunathilake, K. K. D. S. Ranaweera
and H. P. Vasantha Rupasinghe. In Vitro Anti-Inflammatory Properties of
Selected Green Leafy Vegetables. Biomedicines 2018, 6, 107; doi: 10.3390/biomedicines6040107.
END OF DOCUMENTS
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