1.0 INTRODUCTION:
Inflammation is a body response to injury, infection, or
destruction characterized by heat, redness, pain, swelling, and disturbed
physiological functions, caused by physical trauma, noxious chemical, or
microbial agents. It is the body's response to inactivate or destroy the invading
organisms, remove the irritants, and set the stage for tissue repair. It is
triggered by the release of chemical mediators from injured tissue and
migrating cells. The migration of leukocytes from the venous systems to the
site of damage, and the release of cytokines, are known to play a crucial role
in the inflammatory response. These chemicals cause the widening of blood
capillaries (vasodilation) and the permeability of the capillaries. This will
lead to increased blood flow to the injured site.
2.0 METHODOLOGY:
·
Bovine Albumin Denaturation Method
·
The positive control (5ml)
consisted of 0.2 ml of 1% Bovine Serum Albumin (BSA), 2.8 ml of PBS (pH 6.4),
and 2.0 ml of diclofenac sodium at different concentrations (10, 30, and
100μg/ml).
·
The test sample mixture (5 ml)
consisted of 0.2 ml of 1% Bovine Serum Albumin (BSA), 2.8 ml of buffered
phosphate saline (PBS, pH 6.4), and 2.0 ml of different concentrations test
sample (10, 30, and 100 μg/ ml).
·
Negative control samples will
be contained the same amount of Bovine Serum Albumin (BSA) and PBS with 2.0 ml
of distilled water.
·
The mixture will be incubated
at 37°C for 20 min and heated at 51°C for 20 min.
·
The samples will be cooled,
and the absorbance will be taken at 660nm using a spectrophotometer.
·
The experiment will be carried
out in triplicates, and the percentage inhibition will be calculated using the
formula
(%)
Percentage inhibition = Ac – At/Ac × 100
Where
At is the absorbance of the test and Ac is the absorbance
of the control.
3.0 ENDPOINT PARAMETER(S):
·
% Percentage inhibition activity
4.0 REFERENCE(S):
4.1 Douglas Bosco Aidoo, Daniels Konja, Isaac
Tabiri Henneh, and Martins Ekor. Protective Effect of Bergapten against Human
Erythrocyte Hemolysis and Protein Denaturation In Vitro. International Journal
of Inflammation Volume 2021, Article ID 1279359, 7 pages https://doi.org/10.1155/2021/1279359.
4.2 Yesmin, S., Paul, A., Naz, T. et
al. Membrane stabilization as a mechanism of the anti-inflammatory
activity of ethanolic root extract of Choi (Piper chaba). Clin
Phytosci 6, 59 (2020). https://doi.org/10.1186/s40816-020-00207-7
4.3 K. D. P. P. Gunathilake, K. K. D. S. Ranaweera
and H. P. Vasantha Rupasinghe. In Vitro Anti-Inflammatory Properties of
Selected Green Leafy Vegetables. Biomedicines 2018, 6, 107; doi: 10.3390/biomedicines6040107.
END OF DOCUMENTS
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