1.0 PURPOSE
To design the standard operating procedure for the operation of the Automated Cell Counter.
2.0 SCOPE
2.1 The scope applies to the counting and viability measurement of trypan blue stained samples using the Automated Cell Counter and other related works.
4.0 RESPONSIBILITY
4.1 All the users including Scientists and Research persons in the biology department are responsible for the implementation of this SOP and maintaining the Cell Counter.
5.0 DISTRIBUTION
5.1 The Quality Assurance department is responsible to keep SOP ‘Master Copy’ approved through the Quality Manager.
5.2 The copy of ‘Control Copy’ of this SOP is being distributed in the biology department and placed near related Equipment/ Instrument as ‘Display Copy’.
6.0 ABBREVIATIONS
6.1 Abbreviations
6.1.1 SOP : Standard Operating Procedure
6.1.2 µl : Microliter
7.0 DEFINITIONS
7.1 Definitions
7.2.1 The automated Cell Counter accurately counts cells in one simple step using innovative auto-focus technology and a sophisticated cell-counting algorithm. The Cell Counter enables accurate mammalian cell counting in less than 30 seconds.
8.0 PROCEDURE
8.1 Switch on the instrument by pressing the power switch located on the right side at the bottom.
8.2 Handle the counting slides using the edges and avoid touching the optical surface of the slides.
8.3 Pipet 10µl of the cell suspension into the outer opening of either chamber of the counting slide.
8.4 Insert the counting slide into the slide slot. The cell counter will automatically initiate a cell count.
8.5 The count results appear on the Current Count screen as total cell count per ml. If the number of the cells is above or below the specified range, “Value out of range” is displayed on the screen. To see the image, select the View Image key from the Current Count screen.
8.6 The dilution calculator is used to calculate volume adjustments needed to achieve the cell concentration required for the experiment.
8.7 Once the instrument completes the cell count, remove the slide.
8.8 Switch off the instrument after use.
9.0 PRECAUTIONS
9.1 Do not overfill or underfill the chamber. Overfilling the chamber & possibly resulting in accidental spillage of samples inside the instrument could lead to biological contamination of the cell counter.
9.2 Dispose of used slides as biohazardous waste according to the safety regulations.
9.3 Make sure that the cell suspension is thoroughly mixed by pipetting/vortexing. Failure to do so can result in improper sample representation within the loaded counting chamber.
10.0 REFERENCE(S)
10.1 Reference
10.1.1 User manual.
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for Microbiology Laboratory
2. List of All SOPs and Documents for In-vivo Laboratory
3. List of All SOPs and Documents for In-vitro Laboratory
4. List of All SOPs and Documents for Animal House Facility
5. List of All SOPs and Documents for Laboratory Instruments and Equipment
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment