STANDARD OPERATING PROCEDURE FOR EMBEDDING IN HISTOPATHOLOGY

1.0 PURPOSE

1.1 To make a medium for the processed tissue, so that sections from it can be obtained without any distortion or loss of orientation.

2.0 SCOPE

This SOP shall be applicable to embedded tissue.

3.0 RESPONSIBILITY

3.1 Histopathology Department Personnel, Head and Quality Assurance Person.

4.0 DISTRIBUTION

4.1 The Quality Assurance department is responsible for keeping SOP ‘Master Copy’ approved through Quality Assurance Head.

4.2 The copy of ‘Control Copy’ of SOP is being distributed in user departments and placed near related Equipment/Instruments as a Display Copy.

5.0 DEFINITION(S)

5.1 Definition(s)

N/A

6.0 ABBREVIATION(S)

6.1 Abbreviation(s)

6.1.1 SOP: Standard Operating Procedure

6.2.1 No. : Number

6.3.2 Dept. : Department

6.4.3 QA: Quality Assurance Person or Executive

6.5.4 Next Rev: Next Revision

6.6.5 Rev. : Revision/Review

7.0 PROCEDURE

7.1 Instrument details:

7.2 Requirements: Paraffin wax, Moulds, Tissue Embedder, Forceps

7.3 Embedding equipment

7.3.1 Embedding molds are used for casting/shaping liquid paraffin into blocks. There are many types. Stainless steel molds are perhaps the most widely used and are considered ideal for embedding purposes.

7.3.2 Embedding centers are multifunctional units usually equipped with a paraffin dispenser, specimen holding tank, warm plat for orienting the specimen in melted paraffin, and a cold plate for transforming the melted paraffin into a solid block after the specimen has been introduced.

7.4 Paraffin embedding procedure

7.4.1 Using forceps warmed with a commercial forceps warmer to prevent the paraffin from collecting on them, remove a processing cassette that contains the impregnated tissue from the paraffin holding area.

7.4.2 Transfer the mold from the warm plate to the cold plate and the wax will quickly form a thin solid layer on the bottom of the mold .gently press the surface of the tissue to be sectioned against the solid layer which will hold in the desired position. If multiple pieces are to be embedded this process must be carried out rapidly, otherwise, the paraffin will solidify before each piece of tissue can be orientated appropriately.

7.4.3 If an embedding ring or cassette is used, it should be placed firmly on top of the embedding mold at this point. Fill the combined mold and embedding cassette/ring with paraffin and cool immediately by placing the mold on the cold plate of the embedding console the paraffin solidifies in about 15 minutes; the mold is then separated from the embedding cassette. The tissue and solidified wax remains attached to the embedding cassette and ring, forming a paraffin block that is now ready for sectioning

8.0 PRECAUTIONS

8.1 The technician shall embed the tissue while matching the tissue with the grossing log file, matching the number and size of fragments so as to minimize any loss of tissue or any mix-up.

8.2 The technician in charge shall be responsible for the maintenance of the embedding center, concerning the level of the paraffin tank and the temperature of the cold plate and hot plate.

8.3 The forceps must be warmed before picking any fresh tissue for embedding to prevent any carryover of tissue.

8.4 Only one case should be embedded at one time to prevent any mix-up of tissues

8.5 All specimens (sample & reagents/calibrators) used in this test should be considered potentially infectious. Universal precaution (gloves) should be used for handling & disposal of materials during & after use.

8.6 Use soap for routine hand washing.

8.7 Use the biohazard disposal technique.

9.0 REFERENCES

9.1 References

9.1.1 Refer to the manufacturer’s manual for additional information.

END OF THE DOCUMENT

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