To design a Standard Operating Procedure that describes the procedure for the operation and maintenance of the morris water maze.
2.0 SCOPE
This Standard Operating Procedure (SOP) is applicable for the operation, and maintenance of morris water maze Pharmacology studies conducted at the animal house facility.
3.0 RESPONSIBILITY
3.1 All personnel in the Pharmacology Department shall be responsible for implementation and compliance with the SOP.
3.2 Head/Designees/Scientist/Study Directors of the Pharmacology department shall be responsible to ensure the implementation and compliance with the SOP.
4.0 DISTRIBUTION
4.1 The Quality Assurance department is responsible to keep SOP ‘Master Copy’ approved through Quality Assurance Head.
4.2 The copy of the ‘Control Copy’ of SOP is being distributed in the Animal House Facility department and placed near related Equipment/Instruments as ‘Display Copy’.
5.0 DEFINITION(S)
5.1 Definition(s)
5.1.1 Morris water maze is a behavioral procedure mostly used in neuroscience to study spatial learning and memory. It enables learning, memory, and spatial working to be studied with great accuracy, and can also be used to assess damage to particular cortical regions of the brain. It is used by neuroscientists to measure the effect of neurocognitive disorders on spatial learning and possible neural treatments and to test the effect of lesions on the brain in areas.
6.0 ABBREVIATION(S)
6.1 Abbreviation(s)
6.1.1 SOP: Standard Operating Procedure
6.1.2 PPEs: Personal Protective Equipment
7.0 PROCEDURE
7.1 Introduction
7.2 Instrument description
7.2.1 Circular Washtub 4-5 feet (120-150 cm) in diameter with 2 feet (60 cm) high walls.
7.2.2 Height adjustable, circular platform (8 inches diameter).
7.2.3 Stopwatches.
7.2.4 Camera recording System with Software.
7.3 Operations
7.3.1 Fill a 1.5 m diameter, 45 cm deep, Morris water maze with water to a depth of 26.5 cm.
7.3.2 Adjust the water temperature to 25˚C (± 0.5°C).
6.1 Abbreviation(s)
6.1.1 SOP: Standard Operating Procedure
6.1.2 PPEs: Personal Protective Equipment
7.0 PROCEDURE
7.1 Introduction
The Morris water navigation task was conceived by Richard G. Morris (then at the University of St Andrews) in 1981 as an alternative to the radial maze. The test was developed to study spatial learning and how it differed from other forms of associative learning. Originally rats, now more commonly mice were placed in an open pool and the latency to escape was measured for up to six trials a day for 2–14 days.
7.2.1 Circular Washtub 4-5 feet (120-150 cm) in diameter with 2 feet (60 cm) high walls.
7.2.2 Height adjustable, circular platform (8 inches diameter).
7.2.3 Stopwatches.
7.2.4 Camera recording System with Software.
7.3 Operations
7.3.1 Fill a 1.5 m diameter, 45 cm deep, Morris water maze with water to a depth of 26.5 cm.
7.3.2 Adjust the water temperature to 25˚C (± 0.5°C).
7.3.3 Place a circular platform, 25 cm high and 12 cm in diameter, into the tank at a fixed location in the centre of one of four imaginary quadrants.
7.3.4 Immediately prior to behaviour testing, allow the rat an adaptation period of 10-15 minutes (to adapt to white light).
7.3.5 Give the rat a 1-minute rest on the platform before undergoing 3 swim training trials.
7.3.6 During each training trial, remove the rat from the stand and release facing the platform at a distance of 12-18 inches.
7.3.7 Guide the rats/mice to swim to the platform.
7.3.8 A rest period of 30 seconds will be followed by 10 test trials Two sets of 5 trials (3 trials from each of the 3 start positions, with a rest period of 30-45 minutes (in a small heated cage in a different room) separating the two test periods).
7.3.9 During the test trial, release the rat into the water at one of the three different starting positions, facing the wall, and use distal cues (e.g. cabinets, doors, posters, etc.) to navigate to the platform.
7.3.10 The start positions will all be equidistant from the platform and will be located immediately adjacent to the wall in the center of the three quadrants that will not contain the platform.
7.3.11 Allow the rats/mice to swim for up to 1 minute to locate the platform. If the rat fails to find the platform within that time, escape will be assisted.
7.3.12 Measure distance traveled and escapes latency on each trial. Swim speeds, in cm/s, will be calculated by dividing the distance traveled by the escape latency.
7.3.13 After each trial, dry the animal immediately.
7.4 Maintenance
7.4.1 Clean units periodically with a mild disinfectant and a soft cloth.
7.4.2 Any additional maintenance/service should be performed by authorized personnel.
8.0 PRECAUTIONS
8.1 All PPEs like aprons, gloves, masks, head caps, and shoe covers should be worn before using the instrument.
8.2 Press Keys softly.
8.3 Clean the equipment with a dry piece of robe or a lightly moistened one, do not use organic solutions.
8.4 Never use abrasive products or dissolvents.
8.5 Never pour water or liquids on the equipment.
8.6 Keep it clean on its surface; you can usually clean the surface of the device with a wet cloth. Do not use chemical reagents, cleansers or strong scour to clean.
9.0 REFERENCES
9.1 Reference
9.1.1 http://www.nhmrc.gov.au
9.1.2 http://www.adelaide.edu.au/ANZCCART/
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for the Microbiology Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the Animal House Facility
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