1.0 PURPOSE
To design a Standard Operating Procedure that describes the procedure for operation and maintenance of a Stereotaxic injector.
2.0 SCOPE
This Standard Operating Procedure (SOP) is applicable for the assembly and maintenance of digital Stereotaxic and Stereotaxic injectors for studies conducted.
3.0 RESPONSIBILITY
3.1 All personnel in the Pharmacology Department shall be responsible for implementation and compliance with the SOP.
3.2 Head/Designees/Scientists/Study Directors of the Pharmacology department shall be responsible to ensure the implementation and compliance with the SOP.
4.0 DISTRIBUTION
4.1 The Quality Assurance department is responsible to keep SOP ‘Master Copy’ approved through the Quality Assurance.
4.2 The copy of the ‘Control Copy’ of SOP is being distributed in the pharmacology department and placed near related Equipment/ Instrument as Display Copy.
5.0 DEFINITION(S)
5.1 Definition(s)
6.1 Abbreviation(s)
6.1.1 SOP : Standard Operating Procedure
6.1.4 PPEs : Personal Protective Equipment
6.1.5 mm : Millimeter
6.1.6 e.g. : For Example
6.0 PROCEDURE
6.1
Introduction
The innovative design of the Stoelting
Stereotaxic Instrument provides users with the ability to perform surgeries on
both Mice and Rats on the same base. The stereotaxic instrument includes a warmer
ready base, 100 micron 3-axes, left-hand manipulator arm, separate rat and
mouse adaptors, dual-sided (18° and 45°) rat ear bars, triple point mouse ear
bars, and corner clamp probe holder. Available as dual, ultra-precise, digital, or motorized.
Fig 1: Stereotaxic instrument
6.2
Equipment
description
6.2.1
Base plate with rat adapter
6.2.2
Manipulator
6.2.3
Probe holder with mounting adapter
6.2.4
Stereotaxic injector
6.2.5
Ear bars
6.2.6
Controller
6.3
Assembly
6.3.1 The rat adapter assembly (Fig. 2) is shipped and mounted
upside down on the main frame to prevent damage. Loosen the thumbscrew. Flip
the rat adapter assembly right side up, and reinstall the rat adapter (Fig. 3).
Figure
2: For shipping, the rat adapter is
mounted upside down
Figure 3: Reinstall the thumbscrew
6.3.2
To install the manipulator, loosen the turret lock
handle, so that it does not protrude into the mounting hole (Fig. 5). If the
lock is not loosened sufficiently, the turret will not fit over the turret
post.
6.3.3 Align the turret with the turret post (Fig. 5), so that the manipulation assembly slides down into contact with the base of the turret post.
Figure
4: The rat adapter is properly installed.
6.3.4
The orientation of the manipulator can be adjusted in
the horizontal plane. Simply loosen the turret lock handle and rotate the
manipulator assembly to the desired orientation. Use the scale on the base of
the manipulator assembly to properly align it (Fig. 7). Tighten the turret lock
when you are finished.
Figure 5: (Left) Position the turret over the post
and slide it into place
Figure 6: (Right) tighten the turret lock.
Figure 7: Line up the scale
on the turret with the line on the turret post to properly orient the
manipulator assembly in the horizontal plane.
6.3.5
The probe holder is provided pre-assembled with a
mounting clamp that incorporates a V-shaped fitting that couples directly to
the mating ˝V˝ recess on the X-axis of the manipulator. Align the probe holder
with the manipulator and hold it in place (Fig. 8).
6.3.6
Align the thumbscrew with the hole. Then, tighten the
thumbscrew to secure the probe holder to the manipulator assembly (Fig. 9).
Figure 8: (Left) Align the probe holder with the V-shaped notch on the manipulator arm.
Figure 9: (Right) Secure the probe holder with the
thumbscrew.
6.3.7
The ear bars mount into the ear bar channels (Fig. 10).
Loosen the thumb screws. Slide each ear bar into its slot and gently tighten
the thumbscrews enough to secure them without over-tightening them (Fig. 12).
Figure 10: Slide the ear bar into position and
tighten the thumbscrew
6.3.8
The pointed end of the ear bar should face toward the
center, and the ear bars should be installed so that the numbers are right side
up and the increments on the two vernier scale touch each other.
Figure 12: (Right) Use the thumbscrews to secure
the ear bars in place.
6.4
Stereotaxic
injector
The Stoelting Stereotaxic Manual Injector provides an economical means
for any application requiring injecting/withdrawing a calibrated volume under
easy manual control. The Manual Stereotaxic Injector mounts easily on to any
Stoelting Stereotaxic instrument including the Lab Standard, Just for Mouse,
Just for Mice and New Standard. Injection directly through a needle on the
syringe avoids the compliance issues that arise when injections are forced
through plastic tubing.
The injector has a universal adaptor for all microsyringes available at
Stoelting, including the Hamilton 700, 7000, or Gas-Tight Series syringes,
ranging in size from 1 to 100 μl. plumber adaptor allows both infusion and
withdrawal operation. The precise withdrawal mechanism makes it simple to pick
up even one small (amount) dose.
7.1 All PPEs like aprons, gloves, masks, head caps, and shoe covers should be worn before using the instrument.
7.2 Press Keys softly.
7.3 Clean the equipment with a dry piece of robe or a lightly moistened one, do not use organic solutions.
7.4 Never use abrasive products or dissolvent them.
7.5 Never pour water or liquids on the equipment.
7.6 Once you have finished using the equipment turn it off by using the main switch, clean, and check the equipment so that it is in optimal condition when you use it again.
7.7 Do not position the unit so that it is difficult to operate the main disconnect device.
8.0 REFERENCES
8.1 Reference
8.1.1 Refer to the Stoelting Stereotaxic instrument manual.
END OF THE DOCUMENT
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