Standard Operating Procedure (SOP) for Rodent Stereotaxic Surgery
1.0 Purpose:
The purpose of this Standard Operating Procedure (SOP) is to provide guidelines and instructions for performing rodent stereotaxic surgery. This procedure is commonly used in neuroscience research to precisely target specific brain areas for various experimental purposes.
2.0 Scope:
This SOP applies to all personnel involved in performing rodent stereotaxic surgery in the laboratory.
3.0 Responsibilities:
The Principal Investigator (PI) is responsible for ensuring
that all personnel involved in stereotaxic surgery are adequately trained and
follow this SOP.
The surgeon is responsible for performing the surgery in
accordance with this SOP and maintaining aseptic techniques.
The animal care staff is responsible for providing
appropriate pre-and post-operative care to the animals.
4.0 Materials and Equipment:
The stereotaxic instrument with appropriate adapters for
different rodent species
Surgical tools (scalpel, forceps, scissors, etc.)
Anesthetic agent (e.g., isoflurane)
Analgesics for post-operative pain relief
Sterile surgical drapes, gloves, and masks
Sterile saline or artificial cerebrospinal fluid
Sterile sutures or wound clips
A heating pad or lamp for maintaining body temperature
Surgical microscope (optional)
5.0 Procedure:
5.1 Pre-operative Preparation:
Sterilize all surgical instruments and the stereotaxic
apparatus before starting the surgery.
Prepare the surgical area by disinfecting the surgical table
and placing sterile drapes.
Set up the anesthesia system and ensure it is functioning
properly.
Check the animal's health status and verify if it is
suitable for surgery.
Administer pre-operative analgesics and anesthetics as per
approved protocols.
5.2 Surgery:
Place the anesthetized rodent in the stereotaxic apparatus,
ensuring proper alignment and stability.
Maintain anesthesia throughout the surgery using a nose cone
or a mask.
Make a midline incision in the scalp and carefully remove
the skin to expose the skull.
Identify the bregma and adjust the stereotaxic coordinates
according to the desired target region.
Use a drill or a microinjection system to create burr holes
at the planned coordinates.
Lower the desired surgical instrument (e.g., electrode,
cannula) into the brain to the target depth, taking necessary precautions to
avoid damaging critical structures.
Complete the desired procedure (e.g., injection,
stimulation) while monitoring vital signs and adjusting the depth as needed.
After completing the procedure, carefully withdraw the
surgical instrument and close the incision using sutures or wound clips.
Administer post-operative analgesics and monitor the animal
during recovery.
Provide appropriate post-operative care, including warmth,
access to food and water, and monitoring for signs of pain or distress.
6.0 Safety and Biosafety Considerations:
Follow institutional guidelines for working with animals and
ensure compliance with ethical and regulatory requirements.
Use personal protective equipment (PPE) such as gloves,
masks, and lab coats during the procedure.
Maintain aseptic techniques to minimize the risk of
infection.
Properly dispose of all surgical waste and contaminated
materials according to institutional guidelines.
7.0 Documentation and Record-Keeping:
Maintain accurate and detailed records of each surgical
procedure, including animal identification, surgical parameters, and
post-operative care.
Document any deviations from the SOP and report them to the
PI or designated supervisor.
8.0 Training and Qualification:
All personnel involved in rodent stereotaxic surgery must
receive appropriate training from qualified individuals.
Training should cover the principles of stereotaxic surgery,
aseptic techniques, anesthesia administration, and post-operative care.
9.0 References:
Include relevant references or institutional guidelines for
rodent stereotaxic surgery.
10 0 Revision History:
Maintain a revision history of this SOP, noting any updates
or modifications made over time.
Note: This SOP serves as a general guideline for rodent
stereotaxic surgery. It is important to consult institutional guidelines,
ethical regulations, and the specific requirements of your research project
when performing this procedure.
END OF THE DOCUMENT
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