To design a standard operating procedure that describes the operation and maintenance of the Digital Randall Selitto.
2.0 SCOPE
This Standard Operating Procedure (SOP) is applicable for the operation, and maintenance of Digital Randall Selitto for Pharmacology studies.
3.0 RESPONSIBILITY
3.1 All personnel in the Pharmacology Department shall be responsible for the implementation.
3.2 Head/Designees/Scientist/Study Directors of the Pharmacology department shall be responsible to ensure the implementation of the SOP.
4.0 DISTRIBUTION
4.1 The Quality Assurance department is responsible to keep SOP ‘Master Copy’ approved through the Quality Assurance Department.
4.2 The copy of the ‘Control Copy’ of SOP is being distributed in the pharmacology department and placed near related Equipment/ Instrument as Display Copy.
5.0 DEFINITION(S)
5.1 Definition(s)
5.1.1 The Stoelting Digital Randall Selitto is an instrument used for measuring pain thresholds in mice and rats.
6.0 ABBREVIATION(S)
6.1 Abbreviation(s)
6.1.1 SOP : Standard Operating Procedure
6.1.3 PPEs : Personal Protective Equipment
7.0 PROCEDURE
7.1 Introduction
7.1.1 The Stoelting Digital Randall Selitto is a first in the pain field for the Randall Selitto test. Now when measuring pain thresholds in mice and rats you are able to do so hands-free and without the necessity of manual recordings, it is now all digital.
7.2 Instrument description
7.2.1 The system will allow you the researcher to test quickly by operating the test probe with one hand while either holding the rodent in the other hand or by using the rodent sling stand (sold separately).
7.2.2 Maximum applied pressure is stored and displayed on the readout until it is reset by the supplied footswitch or until a larger pressure is applied to the paw.
7.2.3 Paw Pressure can also be used as three (3) separate test units; they are Electronic von Frey, Paw Volume Meter (Plethysmometer) a Stoelting exclusive by adding on either the pressure transducer probe EVF and or the Glass stand for paw volume tests.
7.2.4 Pressure Applicator
7.2.4.1 The pressure transducer in the pressure applicator is very sensitive.
7.2.4.2 The pressure applicator is shipped in a “closed” state to prevent possible damage.
7.2.4.3 To open pressure applicator
7.2.4.3.1 Push the small black rubber eyelet at the end of the probe’s handle all the way in.
7.2.4.3.2 The pressure applicator handle is spring-loaded and will open.
7.2.4.4 To close pressure applicator
7.2.4.4.1 Close the pressure applicator to the point that the black tip of the sensor almost touches the other arm making sure that there is about a 2 mm (1/16”) space (gap).
7.2.4.4.2 While holding the pressure applicator in this position, pull the black eyelet out all the way.
7.2.4.4.3 The pressure applicator is now in the “closed” position.
7.2.4.5 Testing the readiness of the unit
7.2.4.5.1 Place the probe on the supplied probe stand
7.2.4.5.2 Zero the instrument as well as place the supplied test weight (clear, square acrylic with the hole in the center) onto the black cone tip.
7.3 Operation
7.3.1 Connecting Probe and Reset Cable
7.3.1.1 Plug the probe into the socket labeled “MO” on the top left of the unit. This socket is the first socket from the left on the top of the unit.
7.3.1.2 Reset footswitch: Plug the reset cable into the “A2” socket on the top of the unit.
7.3.1.3 Turn on the unit.
7.3.1.4 Main switch shall be on the upper left side of the unit. Each time the unit is turned on it shall program itself.
7.3.1.5 The number “8” will be displayed for a few seconds and will change immediately to a “live” state. In this position whatever force is present at the tip of the probe will be displayed (but for only as long as it is present).
7.3.1.6 Right side of the readout will display “GR” for grams.
7.3.1.7 To ZERO the readout with “GR” on the right: Push the “PROG” button shortly (readout blinks and “C” shows on the right), press “CLEAR”.
7.3.1.8 The reading will show the force applied if the tip is touched and then will return to zero”00.0”. ZERO ON THE UNIT MAY VARY BETWEEN 0 AND 0.1 MAX THIS IS NORMAL and is acceptable and tolerated. The typical deviation is in one direction only; it is about 0.1.
7.3.1.9 MAXIMUM VALUE “MH”- This feature is the “PEAK AND HOLD”. The letters “MH”= (Measured High) are displayed on the right side of the readout and indicate the mode.
7.3.1.10 To enter into the above mode push the FUNCTION button once until “MH” shows. It is possible that the meter shows some peak value at the time of turning the “MH” on.
7.3.1.11 Zeroing the “MH” reading
7.3.1.11.1 Press (short) the button marked “PROGR” on the control panel of the unit until the display starts blinking.
7.3.1.11.2 Display should be blinking, press the “CLEAR” button (If the unit does not (0) zero in “MH” mode you must return to “GR” (GRAM mode): To do so press the “CLEAR” button, the unit should now be in “GR” GRAM mode.
7.3.1.11.3 Press the “PROG” button (display will blink) and press clear to (0) zero the unit.
7.3.1.11.4 Press the “FUNCTION” button to return to the “MH” mode. The readout will return to (0) zero when the “RESET” cable is pressed.
7.3.1.11.5 Your unit can also be zeroed by pressing “PROG” and then “CLEAR”. In “MH” mode the last maximum force applied will be displayed.
7.3.1.11.6 “MH” reading will be stored until it is reset or if a larger force is applied.
7.3.1.11.7 To return to the “GR” reading: Push “M/CLEAR”.
7.3.1.12 CABLE RESET- This cable should be plugged into the “A2” socket. Stepping on the footswitch will reset the “MH” value only.
7.3.1.13 OUTPUT- Baud rate is always 9600, eight data bits (unchangeable), no parity, 1 stop bit. The output cable is plugged into the “A1” socket on top of the unit.
7.3.1.14 Converting from “MH” to “GR” reading
7.3.1.14.1 Press and hold the “M/CLEAR” button.
7.3.1.15 Testing with the supplied clear acrylic test weight
7.3.1.15.1 Place your digital applicator on a table; preferably in the horizontal position. The black cone tip is facing upward. You may need to hold the applicator horizontally to prevent any tipping.
7.3.1.15.2 Then proceed as follows
7.3.1.15.3 Press “Clear” once (the unit will be in “GR” mode).
7.3.1.15.4 Press “PROG” once shortly (value will blink)
7.3.1.15.5 Press “CLEAR” again, and the unit will now display a zero (0) value.
7.3.1.15.6 Place the supplied clear acrylic test weight on the top of the black cone tip.
7.4 Maintenance
7.4.1 Clean units periodically by wiping down with a mild disinfectant and a soft cloth.
7.4.2 Any additional maintenance/service should be performed by authorized personnel.
8.0 PRECAUTIONS
8.1 All PPEs like aprons, gloves, masks, head caps, and shoe covers should be worn before using the instrument.
8.2 Press Keys softly.
8.3 Clean the equipment with a dry piece of robe or a lightly moistened one, do not use organic solutions.
8.4 Never use abrasive products or dissolvents.
8.5 Never pour water or liquids on the equipment.
8.6 Keep the device dry. ln case it wets, please wipe away the water in no time and dry it. The liquid including minerals may corrode the circuits inside.
8.7 Keep it clean on its surface; you can usually clean the surface of the device with a wet cloth. Do not use chemical reagents, cleansers or strong scour to clean.
8.8 If you change the circuits by yourself, the product may not work normally.
8.9 If it displays dismal, maybe the batteries have no charge, and please note the polarity of batteries when you charge the batteries.
8.10 When batteries are not in use for a long time, the batteries may leak to corrode the crust or lines. Used batteries can't be thrown away; please protect the environment.
9.0 REFERENCE
9.1 Reference
9.1.1 Refer to the manufacturer’s manual for additional information.
7.1.1 The Stoelting Digital Randall Selitto is a first in the pain field for the Randall Selitto test. Now when measuring pain thresholds in mice and rats you are able to do so hands-free and without the necessity of manual recordings, it is now all digital.
7.2 Instrument description
7.2.1 The system will allow you the researcher to test quickly by operating the test probe with one hand while either holding the rodent in the other hand or by using the rodent sling stand (sold separately).
7.2.2 Maximum applied pressure is stored and displayed on the readout until it is reset by the supplied footswitch or until a larger pressure is applied to the paw.
7.2.3 Paw Pressure can also be used as three (3) separate test units; they are Electronic von Frey, Paw Volume Meter (Plethysmometer) a Stoelting exclusive by adding on either the pressure transducer probe EVF and or the Glass stand for paw volume tests.
7.2.4 Pressure Applicator
7.2.4.1 The pressure transducer in the pressure applicator is very sensitive.
7.2.4.2 The pressure applicator is shipped in a “closed” state to prevent possible damage.
7.2.4.3 To open pressure applicator
7.2.4.3.1 Push the small black rubber eyelet at the end of the probe’s handle all the way in.
7.2.4.3.2 The pressure applicator handle is spring-loaded and will open.
7.2.4.4 To close pressure applicator
7.2.4.4.1 Close the pressure applicator to the point that the black tip of the sensor almost touches the other arm making sure that there is about a 2 mm (1/16”) space (gap).
7.2.4.4.2 While holding the pressure applicator in this position, pull the black eyelet out all the way.
7.2.4.4.3 The pressure applicator is now in the “closed” position.
7.2.4.5 Testing the readiness of the unit
7.2.4.5.1 Place the probe on the supplied probe stand
7.2.4.5.2 Zero the instrument as well as place the supplied test weight (clear, square acrylic with the hole in the center) onto the black cone tip.
7.3 Operation
7.3.1 Connecting Probe and Reset Cable
7.3.1.1 Plug the probe into the socket labeled “MO” on the top left of the unit. This socket is the first socket from the left on the top of the unit.
7.3.1.2 Reset footswitch: Plug the reset cable into the “A2” socket on the top of the unit.
7.3.1.3 Turn on the unit.
7.3.1.4 Main switch shall be on the upper left side of the unit. Each time the unit is turned on it shall program itself.
7.3.1.5 The number “8” will be displayed for a few seconds and will change immediately to a “live” state. In this position whatever force is present at the tip of the probe will be displayed (but for only as long as it is present).
7.3.1.6 Right side of the readout will display “GR” for grams.
7.3.1.7 To ZERO the readout with “GR” on the right: Push the “PROG” button shortly (readout blinks and “C” shows on the right), press “CLEAR”.
7.3.1.8 The reading will show the force applied if the tip is touched and then will return to zero”00.0”. ZERO ON THE UNIT MAY VARY BETWEEN 0 AND 0.1 MAX THIS IS NORMAL and is acceptable and tolerated. The typical deviation is in one direction only; it is about 0.1.
7.3.1.9 MAXIMUM VALUE “MH”- This feature is the “PEAK AND HOLD”. The letters “MH”= (Measured High) are displayed on the right side of the readout and indicate the mode.
7.3.1.10 To enter into the above mode push the FUNCTION button once until “MH” shows. It is possible that the meter shows some peak value at the time of turning the “MH” on.
7.3.1.11 Zeroing the “MH” reading
7.3.1.11.1 Press (short) the button marked “PROGR” on the control panel of the unit until the display starts blinking.
7.3.1.11.2 Display should be blinking, press the “CLEAR” button (If the unit does not (0) zero in “MH” mode you must return to “GR” (GRAM mode): To do so press the “CLEAR” button, the unit should now be in “GR” GRAM mode.
7.3.1.11.3 Press the “PROG” button (display will blink) and press clear to (0) zero the unit.
7.3.1.11.4 Press the “FUNCTION” button to return to the “MH” mode. The readout will return to (0) zero when the “RESET” cable is pressed.
7.3.1.11.5 Your unit can also be zeroed by pressing “PROG” and then “CLEAR”. In “MH” mode the last maximum force applied will be displayed.
7.3.1.11.6 “MH” reading will be stored until it is reset or if a larger force is applied.
7.3.1.11.7 To return to the “GR” reading: Push “M/CLEAR”.
7.3.1.12 CABLE RESET- This cable should be plugged into the “A2” socket. Stepping on the footswitch will reset the “MH” value only.
7.3.1.13 OUTPUT- Baud rate is always 9600, eight data bits (unchangeable), no parity, 1 stop bit. The output cable is plugged into the “A1” socket on top of the unit.
7.3.1.14 Converting from “MH” to “GR” reading
7.3.1.14.1 Press and hold the “M/CLEAR” button.
7.3.1.15 Testing with the supplied clear acrylic test weight
7.3.1.15.1 Place your digital applicator on a table; preferably in the horizontal position. The black cone tip is facing upward. You may need to hold the applicator horizontally to prevent any tipping.
7.3.1.15.2 Then proceed as follows
7.3.1.15.3 Press “Clear” once (the unit will be in “GR” mode).
7.3.1.15.4 Press “PROG” once shortly (value will blink)
7.3.1.15.5 Press “CLEAR” again, and the unit will now display a zero (0) value.
7.3.1.15.6 Place the supplied clear acrylic test weight on the top of the black cone tip.
7.4 Maintenance
7.4.1 Clean units periodically by wiping down with a mild disinfectant and a soft cloth.
7.4.2 Any additional maintenance/service should be performed by authorized personnel.
8.0 PRECAUTIONS
8.1 All PPEs like aprons, gloves, masks, head caps, and shoe covers should be worn before using the instrument.
8.2 Press Keys softly.
8.3 Clean the equipment with a dry piece of robe or a lightly moistened one, do not use organic solutions.
8.4 Never use abrasive products or dissolvents.
8.5 Never pour water or liquids on the equipment.
8.6 Keep the device dry. ln case it wets, please wipe away the water in no time and dry it. The liquid including minerals may corrode the circuits inside.
8.7 Keep it clean on its surface; you can usually clean the surface of the device with a wet cloth. Do not use chemical reagents, cleansers or strong scour to clean.
8.8 If you change the circuits by yourself, the product may not work normally.
8.9 If it displays dismal, maybe the batteries have no charge, and please note the polarity of batteries when you charge the batteries.
8.10 When batteries are not in use for a long time, the batteries may leak to corrode the crust or lines. Used batteries can't be thrown away; please protect the environment.
9.0 REFERENCE
9.1 Reference
9.1.1 Refer to the manufacturer’s manual for additional information.
END OF THE DOCUMENT
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