The purpose of the document is to establish a general and uniform method for the determination of titratable acidity in whole milk powder.
2.0 SCOPE
This standard testing procedure is suitable for the determination of titratable acidity in whole milk powder samples.
3.0 RESPONSIBILITY
The expert and trained chemist are responsible for following this procedure. The technical manager is responsible for implementing this procedure.
4.0 REQUIREMENTS
4.1 Apparatus
4.1.1 Analytical balance.
4.1.2 Burette with a soda-lime guard tube
4.1.3 Pipettes- 2 ml.
4.1.4 Porcelain Dishes
4.1.5 Stirring Rods, of glass, flattened at one end
4.2 Glassware
4.2.1 Burette: Graduated in 0. l ml and with an accuracy of 0.05 ml
4.2.2 Pipette: capacity 2 ml
4.2.3 Measuring cylinders: capacity 50 ml
4.2.4 Conical flasks, f capacity 100 or 150 ml
4.3 Reagent
4.3.1 Standard Sodium Hydroxide Solution: 0.1M (free from any insoluble sodium carbonate impurities)
4.3.2 Rosaniline Acetate Solution(Bench Solution):
4.3.2.1 Dissolve 0.121 g of rosaniline acetate in approximately 50 mL of rectified spirit, containing 0.5 mL of glacial acetic acid. Makeup to 100 ml with rectified spirit.
4.3.2.2 For the Bench solution, dilute 1 mL of the stock solution onto 500 mL with a mixture of rectified spirit and distilled water in equal proportions by volume.
4.3.2.3 Phenolphthalein solution:
4.3.2.4 Dissolve one gram of phenolphthalein in 100 ml of rectified spirit,
Add 0.1N sodium hydroxide solution until one drop gives a faint pink coloration, dilute with
distilled water to 200ml.
5.0 PROCEDURE
5.1 Weigh accurately about 1.0 g of the sample to each of the two porcelain dishes. Add 10ml of
boiling water to each dish and stirring with the flattened of glass rod until a perfectly smooth liquid is obtained.
5.2 Cool to room temperature Use the contents of one dish as a blank by stirring with 2 ml of bench solution of rosaniline acetate.
5.3 Add 1 ml of phenolphthalein indicator solution of the other dish followed by standard sodium
hydroxide solution introduced drop by drop from the burette until, by comparison, the color matches the pink tint of the blank.
5.4 Stir vigorously throughout the time taken for the complete titration shall not exceed 20 seconds.
5.5 The titration shall be preferably under illumination from a daylight lamp.
6.0CALCULATION
The titratable acidity
(as lactic acid), % by mass = 9AN
M
Where,
A = volume in ml of the
standard sodium north light or under illumination from a daylight
lamp hydroxide required for
titration.
N = normality of the standard sodium hydroxide solution.
M = mass in g of milk powder taken for the test.
7.0REVISION/CHANGE HISTORY8.0ATTACHMENTS
· Appendix-1: FLOW CHART FOR TITRATABLE ACIDITY IN WMP
9.0 REFERENCES
· Annex B of IS 1165:2002; RA 2013; Amendment -2,2014
EAS 27: 2006 UHT Milk
END OF THE DOCUMENT
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