Standard Operating Procedure (SOP) for the analysis of mercury in the bone of rats and mice using the ICP-MS analytical technique, based on the provided procedure:
1.0 Objective: To determine the mercury content in rat and mouse bone samples using ICP-MS (Inductively Coupled Plasma Mass Spectrometry).
2.0 Materials and Reagents:
Bone sample from Rat and Mouse
HNO3 (Nitric Acid) - 60 ml
H2O2 (Hydrogen Peroxide) - 30 ml
HCl (Hydrochloric Acid) - 20.1 ml
3.0 Procedure:
3.1 Sample Preparation:
Collect bone samples from rats or mice.
Ensure that all samples are free of muscular traces.
3.2 Sample Drying:
Place the bone samples in a drying oven set to 37°C.
Allow the samples to dry overnight to remove any moisture.
3.3 Sample Weighing:
After drying, cut each bone sample into small pieces with an
approximate weight of 27-30 mg.
3.4 Sample Digestion:
Prepare a digestion mixture by combining the following
acids:
HNO3 (2 ml/sample)
H2O2 (1 ml/sample)
HCl (0.67 ml/sample)
3.5 Digestion Process:
Add 3.67 ml of the digestion mixture to each bone sample.
Place the samples in containers suitable for acid digestion.
Allow the samples to sit overnight while shaking.
3.6 ICP-MS Analysis:
After overnight digestion, prepare the ICP-MS instrument
according to the manufacturer's guidelines.
Inject the digested bone samples into the ICP-MS instrument
for analysis.
Record the mercury concentration in each sample.
3.7 Safety Precautions:
Perform all steps in a well-ventilated fume hood.
Wear appropriate personal protective equipment, including
lab coats, gloves, and safety goggles.
Handle acids with care and follow safety protocols for their
use.
3.8 Data Analysis:
Analyze the ICP-MS data to determine the concentration of
mercury in each bone sample.
4.0 Reporting:
Prepare a detailed report including sample information,
digestion process, ICP-MS analysis results, and any relevant observations.
5.0 Storage:
Store the digested samples in a designated hazardous waste
container for disposal.
This SOP outlines the steps for the analysis of mercury in bone samples using ICP-MS and includes important safety precautions. Please ensure that all personnel conducting this analysis are trained in laboratory safety procedures and are familiar with the specific equipment and chemicals used in the process.
END OF THE DOCUMENT
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