1.0 Purpose
This Standard Operating Procedure (SOP) outlines the procedures for long-term housing of laboratory animals in an animal facility. Long-term housing is defined as housing for a period of more than 30 days.
This SOP applies to all laboratory personnel who are responsible for the care and housing of laboratory animals.
3.0 Responsibilities
The Principal Investigator (PI) is responsible for ensuring that all long-term housing protocols are scientifically justified and approved by the Institutional Animal Care and Use Committee (IACUC). Laboratory personnel are responsible for following this SOP and implementing long-term housing protocols as approved by the IACUC.
4.0 Procedure
Prepare the animal facility. Ensure that the animal facility is clean and sanitary.
Provide adequate space and enrichment for the animals.
Establish a regular maintenance schedule for the animal facility and equipment.
Receive and quarantine the animals. Quarantine all new animals for at least 14 days before introducing them to the general population.
Monitor the animals for signs of illness or injury during quarantine.
House the animals. Group house compatible animals whenever possible.
Provide the animals with a variety of enrichment items to promote natural behaviors.
Monitor the animals regularly for signs of illness or injury.
Provide care and maintenance. Provide the animals with food and water daily.
Clean the cages and enclosures daily.
Weigh the animals regularly and monitor their body weights for changes.
Euthanize the animals at the end of the study. Euthanize the animals using a humane method approved by the IACUC.
Dispose of the animal carcasses properly.
5.0 Records
The following records must be maintained for all long-term housing protocols: Protocol number
Date of approval by the IACUC
Species and number of animals housed
Duration of the housing
Housing conditions (e.g., cage type, group size, enrichment items provided)
Health status of the animals (including any signs of illness or injury)
Body weights of the animals
Date of euthanasia
Method of euthanasia
6.0 Additional Considerations
Long-term housing of laboratory animals requires a high level of commitment and care.
It is important to provide the animals with a clean, comfortable, and stimulating environment.
The animals should be monitored regularly for signs of illness or injury.
Any health problems should be addressed promptly.
7.0 References
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