Standard Operating Procedure (SOP) for Rodent Ovariectomy
1.0 Purpose
The purpose of this SOP is to provide a detailed guide for performing ovariectomy surgery in rodents. Ovariectomy is a common surgical procedure used to remove the ovaries of female rodents. It is often used to study the effects of estrogen deficiency on various physiological processes.
2.0 Responsibility
This SOP applies to all personnel involved in performing ovariectomy surgery on rodents, including principal investigators (PIs), their staff, veterinary care staff, and any individuals assisting in surgical procedures.
3.0 Materials
3.1. Analgesics (as per Rodent Analgesia SOP) 3.2. Anesthetic: isoflurane 3.3. Sterile ophthalmic ointment 3.4. Electric clipper or depilatory cream 3.5. Sterile and non-sterile gauze 3.6. 70% Alcohol 3.7. Chlorhexidine 2% solution or povidone-iodine solution 3.8. Sterile isotonic saline solution 3.9. Sterile cotton swabs 3.10. Sterile surgical instruments (including forceps, scissors, sutures, and wound clips) 3.11. Dry bead sterilizer 3.12. Absorbable suture 3.13. 7mm or 9mm wound clips (autoclips) 3.14. Heating disc/pad, red heat lamp, or incubator.
4.0 Procedures
Preparation 1.1. Refer to Rodent Surgery SOP for general surgical preparation guidelines. 1.2. Administer analgesia pre-operatively as per Rodent Analgesia SOP. 1.3. Anesthetize the animal using isoflurane according to the Anesthesia SOP. 1.4. Apply ophthalmic ointment to both eyes to prevent corneal desiccation. Reapply as needed.
Hair Removal 2.1. Remove hair over the animal's dorsum, between the rib cage and hip surgical area, using a clipper, depilatory cream, or by plucking. 2.2. Remove loose hair with sterile gauze.
Skin Preparation 3.1. Wipe the skin surface with 70% alcohol followed by 2% chlorhexidine solution or povidone-iodine solution.
Ovariectomy 4.1. Place the animal in sternal recumbency. 4.2. Make a midline incision, approximately 1 cm in length, in the mid-dorsum of the animal. 4.3. Gently separate the skin from the underlying muscle using sterile cotton swabs or a blunt probe. 4.4. Locate each ovary by visualizing a white spot under the muscle on the flanks of the animal. This white spot is the fat pad covering the ovary.
Removal of Ovaries 5.1. For each ovary: 5.1.1. Make a small incision, approximately 0.5 cm in length, directly over one of the white spots (ovaries). 5.1.2. Soak a sterile gauze pad with sterile isotonic saline. 5.1.3. Grasp the fat pad using fine forceps, gently pull it out of the incision, and rest it on the saline-soaked gauze. 5.1.4. Using two fine forceps, gently pull apart the ovary and fat pad from the uterine horn. Alternatively, cauterize the ovary and fat pad to remove it. Set the removed ovary aside. Check for bleeding. 5.1.5. Use sterile cotton-tipped swabs to gently return the uterine horn into the abdominal cavity. 5.1.6. Apply topical local analgesic to the muscle incision. 5.1.7. Hold the edges of the muscle incision together with forceps and use one suture to close the muscle.
Closure 6.1. Apply one drop of topical local analgesic to the skin incision. 6.2. Hold the edges of the skin incision together with forceps and use one wound clip to close the incision. Alternatively, suture the incision.
Post-operative Care 7.1. Disinfect the instruments between each animal by dipping them in a hot glass bead sterilizer for approximately 30 seconds after removing any blood and debris (let cool completely). 7.2. Allow animals to recover in a clean cage. Provide supplemental heat (using a heating disc or pad, heating lamp, or incubator) for approximately 30 minutes and monitor the animals until they have fully recovered before returning them to their housing room.
END OF THE DOCUMENT
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