EVALUATION OF ACTIVITY OF HERBAL FORMULATIONS IN RAT MODEL OF QUINPIROLE-INDUCED COMPULSIVE CHECKING BEHAVIOUR

EVALUATION OF ACTIVITY OF HERBAL FORMULATIONS IN RAT MODEL OF QUINPIROLE-INDUCED COMPULSIVE CHECKING BEHAVIOUR

1.0 TEST SYSTEM DETAILS:

Species : Rattus norvegicus (Rats)

Strain: Sprague Dawley or Wistar

Age: 6-7 weeks

Sex: Male

No. of animals: 10 /Group

Total animals: 60 Males

2.0 TEST ARTICLES DETAILS

HF1: Herbal Formulation-1

3.0 ALLOCATION OF GROUPS:

Group No.	Group Description	Disease disease-inducing agent administered	Treatment administered	Dose Volume and Route
G1	Normal Control	Sterile normal saline, i.p., q.d., 15 injections, twice weekly.	0.5% MC, p.o., b.i.d.	5 ml/kg, p.o.
G2	Disease Control	Quinpirole (0.5 mg/kg)- prepared in Normal saline, 15 injections, twice weekly. To be administered in a volume of 1 ml/kg	0.5% MC, p.o., b.i.d.
G3	Reference Control		Paroxetine: 6 mg/kg, p.o., (Morning) + 0.5% MC (Evening)
G4	Treated with low dose of HB1		HB1: 15-50 mg/kg, b.i.d.  in 0.5% MC
G5	Treated with mid dose of HB1		HB1: 50-150 mg/kg, mg/kg, b.i.d. in 0.5% MC
G6	Treated with high dose 2 of HB1		HB1: 150-500 mg/kg,   b.i.d. in 0.5% MC

Abbreviations: MC-Methyl Cellulose, p.o.-per os. q.d.: quaque die; bid: bis in die

4.0 METHOD:

· Healthy animals will be selected for the study, randomized based on body weight and will be assigned to 6 groups consisting of 10 animals each.

· Before disease induction, rats will be handled for about 2 min daily for 5 days.

· Animals will be induced with obsessive compulsive checking by 15 injections of Quinpirole- 0.5 mg/kg BW, twice weekly, subcutaneously. After 15 min of Quinpirole administration, animals will be placed in an open field and their behaviour will be videotaped continuously throughout a 30 min session.

· Animals of the Group G1 will be designated as sham-control and administered MC, p.o., b.i.d.

· Disease control animals (assigned to group G2) will receive 0.5% MC, p.o., b.i.d.

· Animals of group G3 will be treated with reference drug paroxetine at the dose of 6 mg/kg, p.o., q.d. seven days prior to disease induction.

· Animals of group G4-G6 will be treated with HB1, at different dose levels ranging from 15-500 mg/kg, b.i.d.

· The animals allocated to groups G2-G6 will be administered with 15 injections of Quinpirole- 0.5 mg/kg BW, twice weekly, subcutaneously for induction of obsessive compulsive checking behavior. Animals of G1 will be administered with 15 injections of Normal Saline, subcutaneously.

· Compound administration will be initiated 14 days prior to Quinpirole injection from the day of disease induction.

· Fifteen minutes after each injection of Quinpirole, animals will be placed in an open field and their behaviour will be video-recorded continuously for 30 min. The open field consisted of a glass table subdivided into 25 rectangles (locales) and equipped with 4 Plexiglas boxes at fixed locations. A computer, interfaced with a video recorder, will be used to score behaviour. The following measures will be assessed for each session and rat: 1. total distance travelled; 2. total time of activity/inactivity; 3. frequency of stops at each open field locale; 4. mean duration of return time to a given locale; 5. mean stop duration at a given locale; 6. total duration of stops at a given locale; 7. sequence of visits. The locale with the highest total duration of stops will be individually defined as the home base and compulsive checking behaviour will be analyzed with reference to the home base and in comparison to saline-treated controls. The total distances moved by the rats and the frequency of the freezing behaviour will also be studied.

· Fifty-two days after start of disease induction, animals will be sacrificed under overdose of thiopentone anaesthesia. Immediately after the animal dies, the brain will be collected, fixed and examined for histopathological changes.

5.0 PARAMETERS TO BE EVALUATED:

· Body weight: Twice a week.

· Behavioural assessment of the animals in the open field test,

· Histopathology of brain.

· Estimation of the neurotransmitter levels of glutamate, dopamine, serotonin and GABA in brain.

6.0 REFERENCE(S):

1. Winter, C. et al. High frequency stimulation and temporary inactivation of the subthalamic nucleus reduce quinpirole-induced compulsive checking behavior in rats. Exp. Neurol. 210, 217–228 (2008).

2. Abounoori, M., Maddah, M. M., Akbari, E., Houshmand, G. & Ardeshiri, M. R. The Effect of Orexin Receptor Antagonism on Quinpirole-Induced Compulsive-Like Checking Behavior in Rats. Neurotox. Res. 38, 18–26 (2020).

3. Szechtman, H. et al. Compulsive checking behavior of quinpirole-sensitized rats as an animal model Obsessive-Compulsive Disorder(OCD): Form and control. BMC Neurosci. 2, (2001).

4. Dorfman, A., Szechtman, H. & Eilam, D. Social interaction modulates the intensity of compulsive checking in a rat model of obsessive-compulsive disorder (OCD). Behav. Brain Res. 359, 156–164 (2019).

                                                              END OF THE DOCUMENT  

You may like to read these links: 

1. List of All SOPs and Documents for the Microbiology Laboratory

2. List of All SOPs and Documents for In-vitro Laboratory

3. List of All SOPs and Documents for the In-vivo Laboratory.

4. List of All SOPs and Documents for Clinical Research

5. List of Instruments and Equipment Used In Laboratory Animal Facility

6. Animal  Facility Design - Small Laboratory Animals (Rat, Mice, Rabbit, Guinea Pig)

7. List of Chemicals Needed for In-vivo Laboratory

8. List of Physiological Data for Small Laboratory Animals 

9. Leading Lab-Diet Suppliers in India and Beyond

10. List of All SOPs and Documents for Animal House Facility

SHARE

Owner

Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.

• 
• 
• 
• 
•