EVALUATION OF SPERMATOGENIC EFFECT OF HERBAL FORMULATION IN EXPERIMENTAL ANIMALS.
1.0 TEST SYSTEM DETAILS:
Species : Rattus norvegicus (Rat)
Strain : Sprague Dawley
Age : 4-6 weeks
Body Wight : 150-180 g
Sex : Male
No. of animals : 10 M in each group
Total animals : 50 M + 15F
2.0 ALLOCATION OF GROUPS
*Route
of drug administration will be decided at the time of experiment mainly preferred oral dosing
6
male rats will be taken extra for the randomization & 15 female rats needed
to evaluate male sexual behaviors.
3.0 METHOD:
·
Healthy animals will be selected,
randomized based on body weight and divided into 5 different groups.
·
Animals will be weighed, numbered, and
divided into different groups.
·
The mode of administration will be oral in
dose dependent manner by feeding needle for 28 days.
·
Copulatory Behaviour will be evaluated on
day 29 in black observation box by introducing male animal with female rats for 30 minutes.
·
Number of mounting, intromission and ejaculation
behavior were noted and recorded for all groups.
· Copulatory behavior is a sexual behavior in which a male animal introduces sperm into a female's body. It consists of mounting, intromission, and ejaculation.
4.0 END POINT PARAMETER(S):
·
Effect on weight of sexual organs and
histological studies
·
Effect on male sexual behavior
·
Sperm count
·
Fructose content in seminal vesicles
· Hormone assay (Testosterone)
· Histopathology of Testes and Epididymis
· Number of mounting, intromission and ejaculation behavior (Optional)
5.0 REFERENCE(S):
4.1
Chauhan NS, Dixit VK. Effects of Bryonia laciniosa seeds on sexual-behaviour of male rats. International journal of impotence research. 2010,
22(3), 190-195.
4.2
Suresh S, Prithviraj E and Prakash S.
Dose and time-dependent effects of ethanolic extract of Mucuna puriens Linn.
Seeds on sexual behavior of normal male rats. Journal of Ethnopharmacology.
2009,122(3), 497-501.
END OF THE DOCUMENT
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