Standard Operating Procedure (SOP) for Mounting Media
1.0 OBJECTIVE
- To impregnate the section on the slide with a transparent
medium having a refractive index close to that of glass. This ensures that all
details, including colors, are illuminated and visible.
- The final step in slide preparation involves covering the
tissue section with a thin glass cover slip, making the slide permanent and suitable
for microscopic examination. To affix the cover slip, three types of mounting
media can be used: natural resins, synthetic resins, and aqueous media.
- The laboratory uses synthetic resins, such as DPX, as the
mounting media for routine hematoxylin and eosin preparation and for most
special stains.
2.0 SCOPE
- This SOP is applicable to all types of stains used in the
Histopathology section.
3.0 RESPONSIBILITIES
- **Laboratory Technicians**: Responsible for following this
SOP and ensuring slides are properly mounted.
- **Laboratory Supervisor**: Ensures that the SOP is
followed correctly and updates it as necessary.
- **Quality Control Personnel**: Verifies that the slides
meet quality standards before use in diagnostic procedures.
4.0 MATERIALS AND EQUIPMENT
- Microscope slides
- Cover slips of appropriate size
- Mounting media (e.g., DPX)
- Glass rods
- Xylene
- Filter paper
- Clean gauze
5.0 PROCEDURE
1. **Selection of Cover Slip**:
- Choose the proper
size cover slip for the sections on the slide.
2. **Preparation of Slides**:
- Ensure the slide
is cleared of xylene by placing it onto filter paper.
3. **Application of Mounting Media**:
- Apply the
mounting media (DPX) to the section surface using a glass rod.
4. **Placement of Cover Slip**:
- Roll the cover
slip onto the slide to expel trapped air.
5. **Cleaning**:
- Clear excess
mounting medium from the edges of the cover slip.
- Avoid touching
the main area with fingers to prevent leaving the slide cloudy.
- Clean the back of
the slide with clean gauze.
6.0 QUALITY CONTROL
- Verify that there are no air bubbles or excess mounting
media on the slide.
- Ensure that the tissue section is clearly visible under
the microscope.
- Confirm that the cover slip is securely affixed and the
slide is clean.
7.0 TROUBLESHOOTING
- **Air Bubbles**: If air bubbles are trapped, gently
re-roll the cover slip to expel them.
- **Cloudy Slides**: If the slide is cloudy, ensure it is
cleaned properly and avoid touching the main area with fingers.
8.0 SAFETY CONSIDERATIONS
- Handle all chemicals, such as xylene and mounting media,
with appropriate protective equipment (gloves, goggles, lab coat).
- Work in a well-ventilated area or under a fume hood when
using volatile chemicals.
9.0 REFERENCES
- Histopathology laboratory guidelines
- Manufacturer instructions for mounting media (DPX)
10.0 DOCUMENTATION
- Record the date, type of stain used, and any observations
in the laboratory logbook.
- Maintain a record of any deviations from this SOP and
corrective actions taken.
END OF THE DOCUMENT
1. List of All SOPs and Documents for the Microbiology Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the In-vivo Laboratory.
4. List of All SOPs and Documents for Clinical Research
5. List of Instruments and Equipment Used In Laboratory Animal Facility
6. Animal Facility Design - Small Laboratory Animals (Rat, Mice, Rabbit, Guinea Pig)
7. List of Chemicals Needed for In-vivo Laboratory
8. List of Physiological Data for Small Laboratory Animals
9. Leading Lab-Diet Suppliers in India and Beyond
10. List of All SOPs and Documents for Animal House Facility
11. List of SOPs and Documents for Histopathology Laboratory
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment