Standard Operating Procedure for Manual Knife Sharpening
To establish a uniform procedure for manual knife sharpening.
2.0 Scope
This procedure applies to the Surgical Pathology section of the laboratory, providing guidance to the technician through the knife sharpening process.
3.0 Introduction
Manual knife sharpening is necessary in cases of short supply of microtome blades. Since the results produced by histologic techniques greatly depend on the quality of the blades used to cut sections, it is imperative that each technician knows how to care for and use their knife properly.
4.0 Principle
Not Applicable
5.0 Analytical Measuring Range and Limit of Detection
Not Applicable
6.0 Responsibility
Technical staff and pathologists.
7.0 Abbreviations
None
8.0 Requirements
Instrumentation and Software
- Knives with handle and back
- Macro Abrasive M2 (Coarse) and M3 (Fine)
- Stone
- Leather strop
- Gauze pieces
- Lubricating oil
9.0 Precautions
Not Applicable
10.0 Limitations & Interferences
Not Applicable
11.0 Documentation
Not Applicable
12.0 Instructions
12.1 Pre-Analytical Steps
- Not Applicable
12.2 Analytical Steps
12.2.1 Place the hone on a firm surface and clean it.
12.2.2 Apply a small quantity of lubricating oil to the surface of the hone.
12.2.3 Attach a backing sheath and handle to the knife to maintain the bevel.
12.2.4 Hold the knife at one end with the thumb and forefinger, ensuring the cutting edge faces away from the operator.
12.2.5 Place the knife at one end of the hone and push diagonally forward with the cutting edge leading.
12.2.6 When the knife reaches the other end of the hone, turn it over on its back and pull it steadily towards the operator.
12.2.7 Continue the procedure until the ragged edges are removed.
B. Procedure (Stropping)
12.2.8 Draw the knife with the cutting edge facing the operator in a toe-to-heel direction on the leather belt.
12.2.9 Turn the knife over on its back and bring it back. This action is the opposite of honing.
12.2.10 Examine the knife edge under a microscope to ensure sharpening is perfect.
12.3 Quality Control and Assurance
Not Applicable
12.4 Calibration and Calibration Verification
Not Applicable
12.5 Storage of Samples
Not Applicable
13.0 Reference Range
Not Applicable
14.0 Critical Value
Not Applicable
15.0 Reporting of Results and Interpretation
Not Applicable
16.0 Contingency Plan
Not Applicable
17.0 References
1. List of All SOPs and Documents for the Microbiology Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the In-vivo Laboratory.
4. List of All SOPs and Documents for Clinical Research
5. List of Instruments and Equipment Used In Laboratory Animal Facility
6. Animal Facility Design - Small Laboratory Animals (Rat, Mice, Rabbit, Guinea Pig)
7. List of Chemicals Needed for In-vivo Laboratory
8. List of Physiological Data for Small Laboratory Animals
9. Leading Lab-Diet Suppliers in India and Beyond
10. List of All SOPs and Documents for Animal House Facility
11. List of SOPs and Documents for Histopathology Laboratory
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