SOP FOR RECORDING OF TEMPERATURE AND RELATIVE HUMIDITY (% RH) IN ANIMAL HOUSE FACILITY | STANDARD OPERATING PROCEDURE FOR MEASUREMENT AND RECORDING OF TEMPERATURE AND RELATIVE HUMIDITY (% RH) IN ANIMAL HOUSE FACILITY

 1.0                            OBJECTIVE

1.1                            To lay down a standard procedure to be followed for measurement and recording of temperature and relative humidity (%RH) in Animal House Facility.

2.0                            SCOPE

2.1                          This SOP shall be applicable to measurement and recording of temperature and relative     humidity (%RH) in Animal House Facility.

3.0                            RESPONSIBILITY

3.1                            Head- Animal House Facility

3.2                            Attendant- Animal House Facility

4.0                            DEFINITIONS

4.1                            Nil

5.0                           PROCEDURE

5.1                            Measurement by Digital Thermo-hygrometer

                     


         

5.1.1                     Recording temperature and relative humidity (% RH)

5.1.1.1               The temperature shall be displayed in °C or °F unit. The desired unit shall be selected by switching the “°F/°C” slide switch at the back of the instrument.

5.1.1.2              The upper and lower display shows current temperature and relative humidity respectively.

5.1.1.3               Check the reading daily (preferably at the same time of day) and maintain the record in Format No. F....................

5.1.2                    Recording Maximum and Minimum Temperature and relative humidity (% RH)

5.1.2.1               In order to recall and record the maximum and minimum temperature/relative humidity, Press the “MAX/MIN/CLEAR” key (when the current temperature/ relative humidity is displaying) once to record Maximum Temperature and % RH readings and twice to record Minimum Temperature and % RH of the Day.

5.1.2.2              Press the “MAX/MIN/CLEAR” key again to display the current temperature and relative humidity in the upper and lower LCD display respectively.

5.2                        Measurement by dry and wet bulb Thermo-hygrometer

5.2.1                   Recording temperature

5.2.1.1             Temperature reading in dry thermometer displays the current environmental temperatures temperature shall be displayed in °C.

5.2.2                  Recording %RH

5.2.2.1              A dry and wet bulb hygrometer is made up of two thermometers – a dry bulb and a web bulb thermometer. The wet-bulb thermometer is covered with a damp cloth dipped in water.

5.2.2.2              Fill the water bottle/container attached to the bottom of instrument.

5.2.2.3              Hang the hygrometer (by the loop at the top) on a wall, for instance, for at least five to 10 minutes before taking the measurements.

5.2.2.4              The difference in temperature between the dry bulb and A wet-bulb is used to calculate the relative humidity of the air.

5.2.2.5              To work out the level of humidity:

5.2.2.6               record the dry bulb temperature (e.g. 15 degrees)

5.2.2.7               record the wet-bulb temperature (e.g. 10 degrees)

5.2.2.8               calculate the wet bulb depression (e.g. 15 – 10 = 5 degrees)

5.2.2.9               From the chart as per Annexure No. A…………….., read down from the wet-bulb depression (5) and across from the dry bulb temperature (15) to where both meet. This will give you the relative humidity as a percentage (in this case it will be 49%).

5.2.2.10           Relative humidity of 100 percent is when the air has as much water vapor as it can hold at a particular temperature.

5.2.3                    Check the reading daily (preferably at the same time of day) and maintain the record in Format No. F……………...

6.0                        ENCLOSURES

6.1                        Formats


Sr. No.

Format Title

Format No.

No. of Pages

1

Daily Temperature and Relative Humidity Record

F…………………..

01

 

6.2                            Annexures

Sr. No.

Annexure Title

Annexure number

No. of pages

1

Wet and Dry Bulb Humidity Chart

A…………………

01

7.0                            ABBREVIATIONS

7.1                            SOP                     : Standard Operating Procedure

7.2                            QAD                    : Quality Assurance Department

7.3                            AHF                     : Animal House Facility

8.0                            REFERENCES

8.1                            In-house

8.2                            User Manual

9.0                            REVISION HISTORY

Sr. No.

Change Control No.

Reason for change

 

 

 

                                                                                                                 

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