1.0
OBJECTIVE
To lay
down the procedure for cleaning, operation &
calibration of the colony counter.
2.0
SCOPE
This
SOP shall be applicable for cleaning, operation &
calibration of the colony counter in the Microbiology Laboratory.
3.0
RESPONSIBILITY
3.1
Microbiologists and above shall be responsible for
the preparation and execution of this SOP
3.2
QC & QA Head shall be responsible for the checking and approval of this
SOP.
4.0
ACCOUNTABILITY
Head QC
5.0
PROCEDURE
5.1
Cleaning
5.1.1
Ensure that the power supply to the colony counter is
switched OFF.
5.1.2
Colony counter shall be dedust daily externally with a
clean dry cloth or 70% I.P.A.
5.2
Operation
5.2.1
Ensure that the colony counter r is properly connected
to the power supply.
5.2.2
Now Switch
'ON' the main switch of the
equipment.
5.2.3
The digital display shall show “0000” if the digital display is not showing “0000” then press the reset button, digital the display will show “0000”.
5.2.4
A petri dish shall be placed on the illuminating surface.
5.2.5
Ensure that the magnifying glass is adjusted to view a clear colony present on the Petri
dish.
5.2.6
The lid of the marker pen shall be open and properly press
the tip of the pen on the colonies present on the petri dish and ensure that the digital display shall show you a colony in numbers.
5.3
Calibration of the Instrument:
5.3.1
Ensure
for the operating procedure and after that calibration shall be started.
5.3.2
An
empty petri dish shall be placed on the illuminating surface for calibration.
5.3.3
The
tip of marker pen shall be press 5 times on petri dish and then ensure the number
displayed on the screen, it shall be 0005.
5.3.4
The
RESET button shall be press and the digital display will show “0000”.
5.3.5 The same procedure shall be repeated by pressing the marker pen 10 times, 15 times, 20 times, 25 times, 50 times, and 100 times.
5.3.6
Ensure
that the calibration of colony counter is valid if the number displayed on the screen is equal to the number of times of marker pen pressed.
5.3.7
After
completion of work the reset button shall be press and switch off the mains.
5.3.8
Frequency:
once in a month
6.0
REFERENCE
N.A
7.0
ANNEXURE
|
Annexure No. |
Title of the Annexure |
Format No. |
|
Annexure I |
Calibration record of Colony counter |
|
8.0
ABBREVIATIONS :
SOP : Standard Operating
Procedure
QCD : Quality Control Department
EQP : Equipment
Dept : Department
QC : Quality Control
QA : Quality Assurance
QCD : Quality Control Department
No. : Number
N.A : Not Applicable
END OF DOCUMENT
1. List of All SOPs and Documents for the Microbiology Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the In-vivo Laboratory.
4. List of All SOPs and Documents for Clinical Research
5. List of Instruments and Equipment Used In Laboratory Animal Facility
6. List of All SOPs and Documents for the Animal House Facility
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment