1.0 OBJECTIVE
1.1
To lay down a standard procedure to be followed for Usage and Calibration of Weighing Balance.
2.0
SCOPE
2.1
This Standard Operating Procedure (SOP) shall be applicable for the Usage and Calibration of Weighing
Balance in Animal House Facility.
3.0
RESPONSIBILITY
3.1
Head / Designee –AHF
3.2
All Personnel
involved in Usage and
Calibration of Weighing Balance.
4.0
DEFINITIONS
4.1
Nil
5.0
PROCEDURE
5.1 Installation
5.1.1 Place the balance on a firm and stable foundation/workbench free from vibrations.
5.1.2 The balance should be placed in a room with stabilized temperature and humidity.
5.1.3 The balance should be installed in a place distant from heat sources.
5.1.4 Place the balance on a leveled foundation. Additionally, level the balance following indication of the balance’s level located at the back of balance’s housing. Leveled balance ensures required measuring accuracy.
5.1.5 Before unplugging the balance from mains always turn off the display by pressing ON/OFF key.
5.2
Leveling
5.2.1 On placing the balance in its intended place of use, it should be leveled. Balance current level status is demonstrated on the level located at the back of balance’s housing. In order to level the balance turn its adjustable feet, located on the bottom section of balance’s housing, in a way to make the air bubble of the level indicator move towards the center the small circle marked on the level indicator.
Figure 1: Balance Leveling
5.3 Functions
5.3.1
Startup and Configuration
5.3.1.1
After
plugging the balance in and the software start up, the instrument is ready.
5.3.1.2
It will
operate properly only in the temperature range specified in technical
parameters (see the manual).
5.3.1.3
Before at the start of the weighing procedure, it is recommended to load the balance’s
weighing pan a few times with mass close to balance max capacity,
5.3.1.4
Check
if unloaded balance indicates “precise zero” and whether the measurement is stable
, if not, press /Delete button,
5.3.1.5
Press
UNITS button, to set a measuring unit:
5.3.1.6
[g],
[mg], [ct], and also if enabled in factory menu (non-verified balances) [kg],
[lb], [oz], [ozt], [dwt], [tlh], [tls], [tlt], [tlc] [mom], [gr], [ti], [N],
[baht], [tola].
5.3.2
Zeroing
5.3.2.1
In
order to zero mass indication press
button. The mass indication on the display should equal zero, and precise zero
should appear.
5.3.2.2
Zeroing
process is tantamount to determining new zero point recognized by the balance
as precise zero . Zeroing is possible only in the stable status of display
indication.
5.3.2.3
Caution:
Zeroing the display indication is possible only within the ±2% range of
instrument’s maximal capacity If the zeroed value is above the ±2% of the
maximal capacity, then the software indicates an error message Err2.
5.3.3
Taring
5.3.3.1 In order to determine the net weight of a weighted object, place the object’s container (packaging) on balance’s weighing pan, and on stabilization of measurement result press
5.3.3.2
On
taking off the weighed load and its packaging from instrument’s weighing pan,
the display indicates sum of total tarred mass with a minus sign.
5.3.3.3
The
software also enables assigning tare value to a product from a database. Then,
on product selection from a database, the software automatically uploads data
on tare value for the specific. product.
5.3.3.4
Caution: Taring negative values is impossible. On tarring negative values the
balance responds with an error message Err3. In such case zero balance’s
indication and repeat tarring procedure.
§
Place weighed object on balance’s
weighing pan and read result only on stabilisation of measurement result,
§
Mass indication of a load placed
on balance’s weighing pan can be tarred for multiple Times by pressing →T←/Insert
(pay CAUTION not to exceed maximal capacity of a balance by applying
multiple tare function).
5.3.4
Animal Weighing Mode
5.3.4.1
Working
mode <Animal weighing> enables reliable determining mass of objects in
motion. In principle, these types of objects generate unstable measurement,
thus it requires using a different filtering method of the measurement signal. Select
function “Animal Weighing” from the working mode option on display by the following
method as mentioned in the user manual.
5.3.4.2
Additional setting for animal weighing mode
5.3.4.2.1
Apart
from the standard settings for this mode (described in the weighing mode),
additional settings have been introduced which describe the operation of the
mode.
5.3.4.2.2
Averaging Time – Amount of time during which measurement
records of the weighed samples are analyzed. Obtained data is used to determine
measurement result
5.3.4.2.3
Threshold - It is a value expressed in mass measuring units. In order to start the measurement, the indication has to exceed the value set in the threshold.
5.3.4.2.4
Auto start - Determines start criterion for measurements: whether they are
initiated manually / on pressing a button or selecting START/ or automatically.
When set on <YES> a measurement starts automatically if indication on balance’s
display exceeds the value of set threshold. The following measurement can start
on unloading the weighed object from balance’s pan (the indication has to
return below the value set in threshold) loading a new object on the pan and
exceeding the threshold’s value by the indication.
It should be remembered, however, that before the start of the animal weighing the above-mentioned options should be set on the appropriate values to meet the expectations and the needs of the working conditions.
5.4 Operating Procedure
5.4.1
Ensure
the weighing range before weighing for maintaining sensitivity of the balance.
5.4.2
Ensure
the leveling of balance (air bubble of the level indicator is in center).
5.4.3
Press
the “CAL” button to initiate the internal calibration process.
5.4.4
Switch
the weighing balance to required weighing mode. For taking animal body weight
switch the balance to animal weighing mode as per instruction mentioned in user
manual.
5.4.5
Set the
balance “AUTOSTART” ON or OFF as per requirement.
5.4.6
If
Balance “AUTOSTART” is set to OFF, F key on keyboard will be required for
startup of weighing process (Refer user manual if required).
5.4.7
Place
the empty container on the weighing pan, and gently press “0/T” key to start
the taring/zeroing, wait until balance gets stable. Zero display appears a
shown below and balance is ready for weighing.
5.4.8
5.4.9 Wait till the balance get stabilizes indicated by the pictogram below:
5.4.10
Place the animal inside the tared
container and wait until the balance gets stable.
5.4.11
Wait
for the displayed value to settle and record the measurement.
5.4.12
Switch
off the balance after use.
5.4.13
To
switch off the balance, press and hold the On/Off key.
5.4.14
Clean
the weighing balance immediately after use, if required.
5.4.15
If case
of power failure, weighing activity shall be resumed (on power resumption) after
performing internal calibration (by pressing the key “CAL” ) again.
5.5.1
Tare
function does not work when the balance indicates a minus quantity or zero. In
this case, the balance display will show the error message.
5.5.2
Clearing
the indication is possible only at stabilized display conditions in the range ±2%
of max capacity.
5.5.3
On
pressing ZERO button, the indication is cleared, and the display shows mass
indication zero and stability symbol.
5.5.4
Before
using the balance leveling must be checked.
5.5.5
Do not
put any magnets on the balance pan.
5.5.6
Do not
load the pan with loads exceeding the balance max capacity.
5.5.7
The
balance can be plugged in only by means of the original power supply should be
compatible with the power network voltage. Connect or change additional devices
only when the balance is turned off the power supply.
5.5.8
If
static electricity influences the balance indications, it is necessary to ground
its footing. The grounding screw is at the balance back and is marked with a symbol
5.5.9
In
order to ensure long-lasting use of a balance and reliable measurement of
weighed loads, follow below procedures:
5.5.10
Start
the balance with no load on the weighing pan (permissible value of the load on the
weighing pan on balance start is ±10% of its maximal capacity).
5.5.11
Before
the start of the weighing process or in case of a drastic change of ambient
conditions at a workstation (e.g. ambient temperature change at a workstation
more than 3°C) the balance requires adjusting. The procedure of balance
adjustment is described further in this user manual.
5.5.12
Weighing
balance loading shall be done precisely as per below mentioned figures:
5.5.12.1 Load balance’s weighing pan steadily with no shocks.
5.5.12.2 Place weighed loads centrally on the weighing pan
5.5.12.3 Avoid side loading, in particular, side shocks
5.6 Calibration
5.6.1 Internal Calibration
5.6.1.1
Frequency: Every time before the first weighing on all working days and before
using the balance on weekends.
5.6.1.2
Perform
the internal calibration of the balance by pressing the key <CAL> key.
5.6.1.3
The
display will show internal adjustment and balance will perform the internal calibration
by using the inbuilt weights.
5.6.1.4 Once
the internal calibration is over the balance automatically returns to the
weighing mode.
5.6.1.5 Document
the calibration activity in the respective logbook.
5.6.2 Balance Verification
5.6.2.1
Pick
the standard weights from the weight box and keep it on the pan and note down
the actual weights under linearity.
5.6.2.2
Verify
the balance with the standard calibrated weights. Actual weights should be
within the acceptance limit as per the weight calibration report.
5.6.2.3
For
Balance weight verification take suitable standard weights of 500 mg, 1 g, 10
g, 100 g, 1 kg and 3 kg and weigh it. Document the results as per Format No:
F/SOP/AHF/052/01-00.
5.6.2.4
Balance
verification should be done every time before using balance and every 15 days if
not in use.
5.6.3
Linearity
5.6.3.1
For
Linearity, select 5 to 7 standard calibrated weights within the range of
weighing capacity of balance and record the measurements in Format No. F/SOP/AHF/052/02-00.
5.6.3.2
Graph
shall be plotted between standard calibrated weight is taken and corresponding weight
reading. The graph should be linear.
5.6.3.3
Graph
should be linear. Attach graph in calibration format.
5.6.3.4
Frequency: Every three months
5.6.3.5
Acceptance Criteria: Coefficient
of correlation= Not less than (NLT) -
0.99
5.6.4
Measurement Uncertainty
5.6.4.1
Carefully
take out the standard weight of 200 g (or higher) and put it on the pan.
5.6.4.2
Record
the weight displayed.
5.6.4.3
Again
put the same weight and record.
5.6.4.4
Record
10 such measurements.
5.6.4.5
Calculate
the average (x) and standard deviation (SD) of all 10 measurements.
5.6.4.6
Calculate
the precision i.e.
5.6.4.7
Acceptance
Criteria: Precision NMT 0.1% of actual weight.
5.6.4.8
The
obtained results to be documented as per Format No: F/SOP/AHF/052/02-00.
5.6.5
Frequency: Every three months.
6.0 ENCLOSURES
6.1 Format
|
Sr. No. |
Format Title |
Format No |
No. of Pages |
|
1 |
Balance Verification |
|
01 |
|
2 |
Balance Uncertainty and Linearity |
|
01 |
7.1
SOP : Standard Operating Procedure
7.2
QAD : Quality Assurance Department
7.3 AHF : Animal House Facility
8.0 REFERENCES
8.1
User
Manual
8.2 In-house
9.0 REVISION HISTORY
|
Sr. No. |
Change Control No. |
Reason for change |
|
|
|
|
END OF DOCUMENT
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