1.0 OBJECTIVE
To lay down the procedure for validation of U.V light sterilization.
2.0 SCOPE
This SOP shall be applicable for validation of U.V light sterilization in the Microbiology Lab.
3.0 RESPONSIBILITY
3.1 Microbiologist and above shall be responsible for the preparation and execution of this SOP
3.2 QC & QA Head shall be responsible for the checking and approval of this SOP.
3.3 HOD shall be responsible for the authorization of this SOP.
4.0 ACCOUNTABILITY
Head QC
5.0 PROCEDURE
5.1 Soyabean Casein Digest Agar plates shall be prepared as per SOP No………………..
5.2 Ensure that four soybean casein digest agar plates shall be taken and inoculated with 10-20 cfu of Bacillus subtilis, E.coli, S.aureus, etc.
5.3 Out of these four plates, two plates shall be incubating at 35°- 37°C for 48 hours and marked as control, and the remaining two plates shall be exposed under UV light which is to be validated.
5.4 Exposure time shall be given for one hour by keeping UV Light ON.
5.5 After that the plates shall be incubated at 35°- 37° C for 72 hours.
5.6 Observations of plates shall be done.
5.7 Acceptance Criteria
The number of colonies observed in plates exposed to U V should be 10 times less than the number of colonies observed in control/ unexposed plates.
Frequency: After every 100 burning hours of U V Light.
6.0 REFERENCES
N.A
7.0 ANNEXURE
N.A
8.0 ABBREVIATIONS :
SOP : Standard Operating Procedure
QCD : Quality Control Department
Dept. : Department
QC : Quality Control
QA : Quality Assurance
QCD : Quality Control Department
No. : Number
N.A : Not Applicable
I.P.A : Iso Propyl Alcohol
Lab. : Laboratory
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