Diabetes Induced Erectile Dysfunction Animal Study Model

1. Pathogenesis of Erectile Dysfunction 

Erectile Dysfunction is due to the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP)-PDE5 pathway. NO synthase (NOS) generates NO when phosphorylated by arginine as a neurotransmitter that wheels penile smooth muscle relaxation, resulting in a normal penile erection after sexual stimulation. Diabetes Mellitus can reduce the activity of NOS and the levels of NO and cGMP in the corpus cavernosum of the penis, which may be one of the most important mechanisms of Diabetes induced erectile dysfunction.

2. Diabetes Induction procedure without any mortality in SD rats.

Step-1 : Preparation of 100 ml of 0.1 M sodium citrate buffer solution: 

Use Autoclaved glassware only, to measure the 90 ml distilled water in a glass bottle, keep seperate10 ml same distilled water to make up the sodium citrate buffer solution volume to 100 ml. Weigh citric acid monohydrate 0.525g and Sodium Citrate Tribasic Dihydrate 0.74 g, add 90 ml distilled water mixed it using a magnetic stirrer, measure the pH 4.5 adding the remaining 10 ml separated distilled water and make the volume 100ml, and store the solution in Deep Freezer -80 Celsius for 10 minutes or in -20 Celsius for 15 minutes. Maintain the sodium citrate buffer solution in an icing bucket till the completion of the injection procedure without fail.

Step-2: Preparation of Streptozotocin (STZ) solution in Sod citrate buffer:  Weigh only 120 mg of STZ, and add only in 12 ml of sod. citrate buffer solution, and dissolve the Streptozotocin (Sigma Aldrich) completely in sod. citrate buffer slowly using a vortex mixer. Filter it using a syringe filter, and inject it within ten minutes into all 5 animals. Mix Streptozotocin only 10 minutes before the intraperitoneal injection to the next 5 animal batches of animals. All animals should be induced in batches of 5 animals only, if there is a delay of 10 minutes, then there will be a chance of degradation of Streptozotocin. If Induction fails in some animals, try to re-induce by the same amount of STZ. One more thing keep all animals 12 hrs fasting before Streptozotocin induction.

3. Induction Diabetes confirmation by evaluating Blood Glucose Level

After the Streptozotocin injection, immediately add 5 % dextrose solution to a drinking water bottle and maintain it for 24 hrs. The same day after 4hrs, check blood glucose level using strips and a glucometer. Many animals will show blood glucose levels like non-induced animals’ blood glucose levels, and those animals will give a diabetic range of blood glucose levels the very next day after 24hrs. After 24 hrs. of STZ induction, there may be a change in a few animals that show blood glucose levels below 200 mg/dl. Then, to these animals, you have to re-induced by using the same dose level of Streptozotocin, one day after, keeping the animals on 12 hrs fasting. On the 7^th day check the blood glucose level above 300 mg/dl reading the animals you can confirm they as diabetic.

4. Maintenance of Blood Glucose Level between 300-450mg/dl to induce Erectile Dysfunction.

After diabetes confirmation, blood glucose levels above 500 mg/dl should be maintained between 300-450 mg/dl to induce erectile dysfunction. To maintain glucose levels use Human Actrapid Insulin (Novo nordisk India Private Ltd.) 1 unit for every 100 mg/dl blood glucose level. Maintenance of blood glucose levels between 300 to 450 mg is compulsory to induce erectile dysfunction, moreover, blood glucose levels above 500 mg/dl may be dangerous to animals and may cause often death in a long study (of more than 28 days). 

ALLOXAN STREPTOZOTOCIN THEIR MECHANISM OF ACTION IN DIABETES INDUCTION

Simplified diagram of the neurobiological circuits of the lordosis sexual reflex

                                  END OF DOCUMENT

You may like to read these links:   

1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agent, Standard or Reference Drug, and related parameters.

4. List of OECD Guidelines or Toxicological Studies

5. List of Pharmacopeial Tests Quality Control Tests or Batch Testing Parameters (In-vivo)

6. EVALUATION OF PROTECTIVE EFFECT OF TEST SAMPLE IN SURGICALLY INDUCED NEUROPATHY PAIN USING CHRONIC CONSTRUCTIVE INJURY (CCI) MODEL IN EXPERIMENTAL RAT

7. EVALUATION OF PROTECTIVE EFFECT OF TEST SAMPLE IN PACLITAXEL-INDUCED NEUROPATHY PAIN MODEL IN EXPERIMENTAL RAT

8. EVALUATION OF PROTECTIVE EFFECT OF TEST SAMPLE STREPTOZOTOCIN-INDUCED DIABETIC NEUROPATHY PAIN MODEL IN EXPERIMENTAL RAT

9. What are the points need to be considered before starting a diabetic wound healing study on rabbits?

SHARE

Owner

Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.

• 
• 
• 
• 
•