2.0 RESPONSIBILITY: It is responsibility of Animal house personals to clean the cages, racks and water bottle at prescribed interval or as per the requirement. The Animal house in-charge should supervise the daily cleaning and washing activity.
3.0 PROCEDURE:
3.1 FOR WASHING THE ANIMAL CAGES AND RACKS:
3.1.1 All small animals were transferred to clean cages at least twice a week or more often if needed.
3.1.2 Wire stainless steel tops were changed and sanitized at least once in a month.
3.1.3 Racks in use were washed once in each quarter of a year. All racks were wiped once in a week. Carts and racks were dusted daily or as often as necessary.
3.1.4 A detergent-disinfectant was used to sanitize racks and carts.
3.1.5 Shoe cover, head cover was worn while working or trafficking through the dirty side of cage and rack wash.
3.1.6 Dirty cages and materials were organized to facilitate cleaning and safe operation of equipment.
3.1.7 The interior and exterior work surfaces on bedding disposal cabinet and other equipments were cleaned disinfectant.
3.1.8 Before starting the cleaning work, it was ensured that the water supply was totally stopped. All water supply inlets and outlets were closed.
3.1.9 It was insured that the tank was clean. One rinse of water was given and then was allowed to drain.
3.1.10 Now Sodium hypochlorite was added to water tank for the washing of cages and racks.
3.2 WATER BOTTLES CLEANING:
3.2.1 Water bottles offering aqua guard clean water, sipper nozzles stopper and other equipment were cleaned regularly thrice a week with water having a temperature of about 82.20C (1800F) and chemical agent like Sodium Hypochlorite to destroy pathogenic organism.
3.2.2 All animal cages and water bottles were sterilized by autoclave at 1200C temperature, 15 lbs pressure for 45 minutes weekly.
3.2.3 Assessment of cleanliness, sanitation and hygiene in animal house by visual monitoring, microbiologic monitoring (swab analysis) was done from time to time.
3.2.4 UV sterilized clean feed was offered to animals and feeding time was recorded in the animal house register.
3.2.5 All the cages were then dipped into water tank for cleaning for approximately 45 minutes.
3.2.6 After the cages had been dumped and rinsed, the cages were put into the autoclave for sterilization for 30 minutes at 120 0C.
3.2.7 All rodent cages (cage bottom, wire bar lids, filter tops, cage card holders, etc.) were washed with water that was autoclaved prior to the use. Affix temperature indicator to autoclave load.
4.0 PRECAUTIONS:
4.1 Be careful while handling the animals when washing the racks and cages.
4.2 Be careful while handling the equipment.
5.0 REFERENCES:
5.1 Cooper DM, McIver R, Bianco R. The thin blue line: A review and discussion of aseptic technique and postprocedural infections in rodents. Contemp Top Lab Anim Sci. 2000;39:27–32. [PubMed]
5.2 Cunliffe-Beamer TL. Biomethodology and surgical techniques. The Mouse in Biomedical Research, vol III: Normative Biology, Immunology and Husbandry. Foster HL, Small JD, Fox JG, editors. New York: Academic Press; 1983. pp. 419–420.
5.3 Cunliffe-Beamer TL. Surgical techniques. Guidelines for the Well-Being of Rodents in Research. Guttman HN, editor. Bethesda, MD: Scientists Center for Animal Welfare; 1990. pp. 80–85.
5.4 Cunliffe-Beamer TL. Applying principles of aseptic surgery to rodents. AWIC Newsletter. 1993;4:3–6.
5.5 Danneman PJ, Stein S, Walshaw SO. Humane and practical implications of using carbon dioxide mixed with oxygen for anesthesia or euthanasia of rats. Lab Anim Sci. 1997;47:376–385. [PubMed]
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