2.0 RESPONSIBILITY: It is the responsibility of the Animal care personnel and Animal House in-charge.
NOTE: Laboratory animals are very sensitive to their living conditions. It is important that they shall be housed in an isolated building located as far away from human habitations as possible and not exposed to dust, smoke, noise, wild rodents, insects and birds.
3.0 ENVIRONMENTAL CONDITION:
3.1 TEMPERATURE AND HUMIDITY CONTROL:
3.1.1 Animal room temperature should be carefully controlled and continuously monitored by instruments that are checked at least twice daily. Once at 9:30 AM for minimum temperature and then at 3:30 PM for maximum temperature.
3.1.2 Air conditioning is an effective means of regulating these environmental parameters for laboratory animals. Temperature and humidity control prevents variations due to changing climatic conditions or differences. Ideally, capability should be provided to allow variations within the range of approximately 22±3 °C, which includes the temperature ranges usually recommended for common laboratory animals.
3.1.3 The relative humidity should be controllable within the range of 30% to 70% throughout the year
3.2 VENTILATION:
3.2.1 The ventilation rate of the room should be 15-20 changes of fresh or conditioned air per hour distributed throughout the room are normally adequate.
3.2.2 The use of HEPA-filtered (high-efficiency particulate air-filtered) recycled air without gaseous filtration (such as with activated-charcoal filters) can be used but only in limited applications, provided that room air is mixed with at least 50% fresh air (that is, the supply air does not exceed 50% recycled air).
3.3 LIGHT CYCLE & INTENSITY:
3.3.1 Intensity – 250-400 lux at bench level should be measured at monthly intervals. The intensity of light will be measured monthly with the help of a lux meter.
3.3.2 Photoperiod - Daily light cycle of 12:12 hours.
3.3.3 A time-controlled lighting system should be used to ensure a regular diurnal lighting cycle wherever required. Emergency power should be available in the event of power failure.
3.4 NOISE LEVEL:
3.4.1 Sound level should be measured with the help of a digital sound meter.
3.4.2 Exposure to sound should not be louder than 85 dB.
4.0 PROCEDURE:
4.1 The animal care personnel maintained the record and noted the exact temperature and humidity.
4.2 The animal house in charge cross-checked the data provided by the animal care personnel.
4.3 The 12 hours light and dark cycle were strictly maintained by the animal care personnel.
4.4 There was no loud sound made in the animal house facility.
5.0 PRECAUTIONS:
5.1 The animal house personnel should carefully keep a daily record of the temperature, humidity, and day-light cycle.
5.2 The animal house in charge needs to give a surprise check from time to time to ensure that the record is being maintained.
6.0 REFERENCES:
6.1 Chamberlain GV, Houang E. Trial of the use of masks in the gynecological operating theatre. Ann R Coll Surg Engl. 1984;66:432–433. [PMC free article] [PubMed]
6.2 Clifford CB, Watson J. Old enemies, still with us after all these years. ILARJILAR J. 2008;49:291–302.[PubMed]
6.3 Coderre TJ, Katz J, Vaccarino AL, Melzack R. Contribution of central neuroplasticity to pathological pain: Review of clinical and experimental evidence. Pain. 1993;52:259–285. [PubMed]
6.4 Conlee KM, Stephens ML, Rowan AN, King LA. Carbon dioxide for euthanasia: Concerns regarding pain and distress, with special reference to mice and rats. Lab Anim (NY) 2005;39:137–161. [PubMed]
6.5 Conour LA, Murray KA, Brown MJ. Preparation of animals for research: Issues to consider for rodents and rabbits. ILAR J. 2006;47:283–293. [PubMed]
END OF THE DOCUMENT
You may like to read these links:
1. List of In-vivo laboratory Vendors (Capex)
2. List of SOPs and Documents for CPCSEA
3. List of All SOPs and Documents for Laboratory Instruments and Equipment
4. List of All SOPs and Documents for the Animal House Facility
5. List of All SOPs and Documents for the In-vivo Laboratory
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