2.0 RESPONSIBILITY: It is the responsibility of Principal Investigator (PI), Animal house In-charge and Animals Care Personals.
3.0 REQUIREMENTS:
3.1 Carbon Dioxide asphyxiation assembly
3.2 Pentobarbitone/Ketamine
3.3 Scissors
3.4 Scalpels/blades
3.5 Forceps
3.6 Personal Protection Equipment (PPE)
3.7 For tissue collection
3.7.1 10% neutral buffered formalin
3.7.2 Fixative of choice unless otherwise specified by study director
3.7.3 Volume should be 10 times the volume of immersed tissue
3.7.4 Leak proof wet tissue container
3.7.5 Size compatible with 10:1 formalin/tissue ratio
3.7.6 If blood sampling is required, blood should be collected as follows:
3.7.6.1 Purple top tube (EDTA)- whole blood/plasma
3.7.6.2 Red/Grey top tube or microtainer (serum separator)- serum
3.7.6.3 Red top tube (plain) or centrifuge tube – serum
3.7.6.4 Syringe- determined by amount needed
3.7.6.5 Micro capillary tubes
3.8 Labels
3.8.1 Animal identification number
3.8.2 Study number
3.8.3 Date
3.9 Necropsy form- description should be
3.9.1 Accurate and brief
3.9.2 Tissue required should be accounted for
3.9.3 Appropriate entries for
3.9.4 Tissue examination, saved and/or fixed
3.9.5 Assurance of animal identification
4.0 PROCEDURE:
4.1 Animal necropsy should be performed in areas specially designated for that purpose.
4.2 Stainless steel tray having wax was used for necropsy and was de-contaminated after each use.
4.3 Necropsy was done in a manner that avoids the occurrence of artifacts and change in the tissue collection.
4.4 Animals that were not to be necropsied were immediately identified and refrigerated and the on-duty Research staff was notified.
4.5 Proper personal protective equipment and attire was worn when performing necropsy.
4.5.1 Disposable gloves, shoe cover and gown were worn when necropsy was being conducted.
4.5.2 Full protective clothing was worn when the animals to be necropsied were infected with bio hazardous materials or chemical carcinogens (double gloves, cap, disposable gown, shoe covers, mask and eye protection).
4.6 Specimens for microbiological assessment were collected using sterile instruments and aseptic technique.
4.7 All specimens, whether collected for submission to a laboratory for analysis or for archival purposes were placed in a suitable container and labeled with Principle Investigator, animal ID and date of collection. Besides this the tissue or sample collected are appropriate and the fixative or specific storage requirements were mentioned if found necessary.
4.8 All tissue submitted for histopathological assessment by a pathologist for studies had all the necessary information to completely identify the specimen, including the PI, animal ID, date of collection, tissue submitted, fixative used or specific storage requirements, any special laboratory test or procedures requested and the name of person conducting the necropsy.
5.0 PRECAUTIONS:
5.1 Carefully label the samples (blood or tissue).
5.2 All the details should be thoroughly checked by the PI and then the Quality Assurance Personal.
5.3 All the equipment's used for necropsy should be washed, cleaned and dried before the start of the necropsy.
5.4 Paraffin wax trays should be cleaned thoroughly to remove the blood stains and other things and should be ready for reuse.
6.0 REFERENCES:
6.1 Feldman, D.B. and Seely J.C., Necropsy Guide: Rodents and the rabbit. CRC Press Inc., Boca Raton FI, 1998.
6.2 Pathology of domestic animals 3rd ed., vols. 1-3 Academic Press Inc., 1985.
END OF THE DOCUMENT
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1. List of In-vivo laboratory Vendors (Capex)
2. List of SOPs and Documents for CPCSEA
3. List of All SOPs and Documents for Laboratory Instruments and Equipment
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