EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING ACETIC ACID INDUCED WRITHING TEST IN EXPERIMENTAL ANIMALS

STUDY PROTOCOL

EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING ACETIC ACID INDUCED WRITHING TEST IN EXPERIMENTAL ANIMALS

Introduction

In this study model, used writhing test is a kind of chemical method used to induce pain of peripheral origin by injecting an irritant principle like acetic acid in mice. Analgesic activity of the test compound is incidental from decrease in the frequency of writhings. Acetic acid induces the inflammatory response in the abdominal cavity, with consequent effect activate the nociceptors.

1.0  TEST SYSTEM DETAILS:

Species             : Mus musculus

Strain                : Swiss albino

Age                   : 6-10 weeks

Body Wight        : 20-25 g

Sex                   : Male

No. of animals    : 8 /Group

 

2.0  ALLOCATION OF GROUPS:

 

Groups	Treatment	Dose; ROA	No. of Animals
G1	Normal Control	Normal saline or 0.25% Na-CMC	8
G2	Reference Drug- Indomethacin	10 mpk; p.o.	8
G3	Test Compound I	X mpk; p.o.	8
G4	Test Compound I	XX mpk; p.o.	8
G5	Test Compound II	X mpk; p.o.	8
G6	Test Compound II	XX mpk; p.o.	8

Extra 5 animals will be taken, considering animal screening based on the basal latency time

 

3.0 METHOD:

·       Animals shall be procured from the CPCSEA authorized vendor.

·       Animals shall be quarantined for 1 week as per the in house SOP.

·       Healthy animals will be selected, randomized based on body weight and divided into 6 different groups consisting of 8 animals each.

·       Group G1 animal will be treated as normal control and treated with normal saline or Na- CMC.

·       Animals of group G2 will be treated orally with Indomethacin at the dose of 10mpk, P.O.

·       Group G3, G4, G5 and G6 animals will be treated with test compound at different dose levels.

·       Thirty minutes later, acetic acid (0.7%, 10 ml/kg, i.p.) will be administered to each mouse to induce abdominal contraction known as writhing.

·       After an interval of 5 min, number of writhes for each group was counted for 10 min and recorded.

·       The number of writhes of test groups at different dose levels, and standard were compared with the control.

 

4.0 END POINT PARAMETER(S):

·       No. of Writhes

·       % Analgesic activity =  Mean no. of writhes of test compound – Mean no. of writhes of control/ Mean no. of writhes of control*100

5.0  REFERENCE(S):

5.1 R. N. Suresha, Siddamma Amoghimath, P. S. Vaibhavi, S. L. Shruthi, M. K. Jayanthi, and H. L. Kalabharathi. Evaluation of analgesic activity of perindopril in albino mice. J Adv Pharm Technol Res. 2014 Jul-Sep; 5(3): 129–133.

5.2  Koster R, Anderson M, De-Beer EJ. Acetic acid for analgesic screening. Fed Proc. 1959;   18:412–8.

                                    END OF DOCUMENT

You may like to read theses links:   

1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agent, Standard or Reference Drug and related parameters.

4.List of OECD Guidelines or Toxicological Studies

5.List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

6.EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING HOT PLATE TEST IN EXPERIMENTAL ANIMALS

SHARE

Owner

Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.

• 
• 
• 
• 
•