EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING HOT PLATE TEST IN EXPERIMENTAL ANIMALS

 

EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING HOT PLATE TEST IN EXPERIMENTAL ANIMALS

1.0  TEST SYSTEM DETAILS:

Species             : Mus musculus

Strain                : Swiss albino

Age                   : 8-10 weeks

Body Wight        : 20-25 g

Sex                   : Male

No. of animals    : 8/Group

 

2.0  ALLOCATION OF GROUPS:

 

Groups	Treatment	Dose; ROA	No. of Animals
G1	Normal Control	Normal saline or 0.25% Na-CMC	8
G2	Reference Drug- Tramadol	40 mpk; i.p.	8
G3	Test Compound I	X mpk; p.o.	8
G4	Test Compound I	XX mpk; p.o.	8
G5	Test Compound II	X mpk; p.o.	8
G6	Test Compound II	XX mpk; p.o.	8

Extra 5 animals will be taken, considering animal screening based on the basal latency time

 

3.0 METHOD:

·       Animals shall be procured from the CPCSEA authorized vendor.

·       Animals shall be quarantined for 1 week as per the in house SOP.

·       Healthy animals will be selected, randomized based on body weight and divided into 6 different groups consisting of 8 animals each.

·       Group G1 animal will be treated as normal control and treated with normal saline or Na- CMC.

·       Animals of group G2 will be treated with tramadol at the dose of 40 mg/kg, i.p.

·       Group G3, G4, G5 and G6 animals will be treated with test compounds at different dose levels.

·       Pain stimulus will be produced by placing the animals on hot-plate maintained at the temperature of 55 ± 0.5°C.

·       Paw licking or jumping off the plate will be considered as response to pain stimulus.

·       Reaction time for each group will be recorded at 0, 30, 60, 90 and 120 min during the observation period.

·       To avoid any possible accidental paw damage, a cut-off point of 15 sec will be considered.

·       Reaction time of the test and standard will be compared with the control.

4.0 END POINT PARAMETER(S):

·       Reaction Time

·  % Analgesic activity = Mean reaction time of control – Mean reaction time of test/Mean reaction time of control)*100

5.0  REFERENCE(S):

5.1 Himangsu Mondal,  Sanjib Saha, Khalijah Awang,  Hemayet Hossain, Abdulwali Ablat, Md Khirul Islam, Ismet Ara Jahan,  Samir K Sadhu, Md Golam Hossain,  Jamil A Shilpi and   Shaikh J Uddin. Central-stimulating and analgesic activity of the ethanolic extract of Alternanthera sessilis in mice. BMC Complementary and Alternative Medicine. 201414:398.

5.2 Menéndez, L., Andrés-Trelles, F., Hidalgo, A., Bahamonde, A., 1993. Involvement of spinal k opioid receptors in a type of footshock-induced analgesia in mice. Brain Research 611, 264–271.

5.3 Sylvia Arraua, Carla Delportea,∗, Carlos Cartagenaa, Maité Rodríguez-Díaz a, Patricia González b, Ximena Silva b, Bruce K. Cassels c, Hugo F. Miranda d. Antinociceptive activity of Quillaja saponaria Mol. saponin extract, quillaic acid and derivatives in mice. Journal of Ethnopharmacology 133 (2011) 164–167.

                                END OF DOCUMENT

You may like to read theses links:   

1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agent, Standard or Reference Drug and related parameters.

4.List of OECD Guidelines or Toxicological Studies

5.List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

6.EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING FORMALIN TEST IN EXPERIMENTAL ANIMALS

SHARE

Owner

Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.

• 
• 
• 
• 
•