EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING TAIL FLICK TEST IN EXPERIMENTAL ANIMALS

                                                                STDUY PROTOCOL

EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING TAIL FLICK TEST IN EXPERIMENTAL ANIMALS

1.0 INTRODUCTION

Tail-Flick Test is a nociceptive essay based on the measurement of the latency of the avoidance response to thermal stimulus in animals. Generally, a thermal stimulus is put on the tail, when the animal feels uncomforted, it reacts by a abrupt tail movement. This test also known as D'Armour & Smith Test.

2.0 TEST SYSTEM DETAILS:

Species             : Mus musculus

Strain                : Swiss albino

Age                   : 6-10 weeks

Body Wight        : 20-25 g

Sex                   : Male

No. of animals    : 8/Group

 

3.0 ALLOCATION OF GROUPS:

Groups	Treatment	Dose; ROA	No. of Animals
G1	Normal Control	Normal saline or 0.25% Na-CMC	8
G2	Reference Drug- Indomethacin	10 mpk; p.o.	8
G3	Test Compound-I	X mpk; p.o.	8
G4	Test Compound-I	XX mpk; p.o.	8
G5	Test Compound-II	X mpk; p.o.	8
G6	Test Compound-II	XX mpk; p.o.	8

Extra 5 animals will be taken considering animal screening based on the basal latency time

3.0 METHOD:

· Animals shall be procured from the CPCSEA authorized vendor.

· Animals shall be quarantined for 1 week as per the in house SOP.

· Healthy animals will be selected, randomized based on body weight and divided into 6 different groups consisting of 8 animals each.

· Group G1 animal will be treated as normal control and treated with normal saline or Na- CMC.

· Animals of group G2 will be treated orally with indomethacin at the dose of 10mpk, p.o.

· Group G3, G4, G5 and G6 animals will be treated with test compound at different dose levels.

· Thirty minutes later, a radiant heat, automatic tail flick algesiometer will be used to measure response latencies.

· The light beam will be focused on the animal’s tail about 4 cm from the tip and the intensity will be adjusted so that baseline readings will be between 2 and 3s.

· An 8 s cut-off time (Toff) will be imposed to avoid damage to the tail.

· After 30 min of the drug treatment, all the animals will be subjected to the tail flick latency measurement at 60, 120, 280 min.

· The number of tail flick latency of the test groups at different dose levels, and standard will be compared with the control.


5.0 END POINT PARAMETER(S):

· No. Tail flick latencies

· % Maximum Possible Effect = 100 X(L2-L1)/(Toff- L1)

where L2 is the post drug latency and L1 is the pre drug latency or control reaction time.


6.0 REFERENCE(S):

5.1 Sylvia Arraua, Carla Delportea, Carlos Cartagenaa, Maité Rodríguez-Díaz a, Patricia González b, Ximena Silva b, Bruce K. Cassels c, Hugo F. Miranda d. Antinociceptive activity of Quillaja saponaria Mol. saponin extract, quillaic acid and derivatives in mice. Journal of Ethnopharmacology 133 (2011) 164–167.

5.2 Le Bars, D. Gozariu, M. Cadden, S.W. 2001. Animal models of nociception. Pharmacology Review 53, 597–652.

                                         END OF DOCUMENT

You may like to read theses links:   

1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agent, Standard or Reference Drug and related parameters.

4.List of OECD Guidelines or Toxicological Studies

5.List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

6.EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING FORMALIN TEST IN EXPERIMENTAL ANIMALS

7.EVALUATION OF ANALGESIC POTENTIAL OF TEST COMPOUND(S) USING HOT PLATE TEST IN EXPERIMENTAL ANIMALS

8.STANDARD OPERATING PROCEDURE FOR OPERATION, MAINTENANCE OF TAIL FLICK UNIT

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