EVALUATION OF ANXIOLYTIC POTENTIAL OF TEST COMPOUND USING ELEVATED PLUS MAZE TEST IN EXPERIMENTAL MICE

 

EVALUATION OF ANXIOLYTIC POTENTIAL OF TEST COMPOUND USING ELEVATED PLUS MAZE TEST IN EXPERIMENTAL MICE

1.0  INTRODUCTION:

Anxiety is a central nervous system (CNS) disorder with negative emotional state, causing uneasiness, fear, etc. in response to factors perceived from internal or external sources. There are four neurotransmitters playing key roles in mood regulations, which are norepinephrine, gamma-aminobutyric acid (GABA), serotonin, and dopamine. Imbalance between these neurotransmitters posses several CNS disorder and among them one is anxiety.

 

2.0  TEST SYSTEM DETAILS:

Species             : Mus musculus (mouse)

Strain                : Swiss albino

Age                   : 7-8 weeks

Body Wight        : 25-30g g

Sex                   : Male

No. of animals    : 8 /Group (Total=40)

 

3.0  ALLOCATION OF GROUPS:

Groups	Treatment	Dose; ROA (p.o.)	No. of Animals
G1	Normal Control	Normal saline or 0.25% Na-CMC	8
G2	Reference Drug- Diazepam tablet	3 mpk; p.o.	8
G3	Test Compound 1	X mpk	8
G4	Test Compound 2	X mpk	8

 

4.0 METHODOLOGY:

·       The animals will be acclimatized for at least 5 days before they will tested and will be randomized on the basis of body weight into different experimental groups (n=8).

·       Group G1 animals will be served as normal control and administered 0.25% Na-CMC; p.o.

·       Group G2 animals will be served as reference control and administered diazepam at the dose of 3 mg/kg; p.o.

·       Similarly, animals of group G3 & G4 will be administered with test compound at different dose levels.

·       All drugs and vehicle will be administrated only once per day via gastric intubation for 7 consecutive days. The tests will be conducted 1 h after the last drug treatment.

·       Body weight of the animals will be recorded every week/day.

 

4.1 Evaluation of Anxiolytic activity (Elevated Plus Maze Test)

·       The test apparatus consisted of two enclosed arms (length 30 cm × width 5 cm × height 15 cm), two open arms (length 30 cm × width 5 cm), and a central platform (5 cm × 5 cm).

·       The maze will be elevated 45 cm above the floor level and mice will be placed individually into the center of the maze, facing one of the open arms for 5 min.

·       During the 5 min, the total number of entries & time spent in each type of arm (Open & closed) will be recorded.

·       Entry into an arm will be defined as when mice placed all four feet into the arm completely.

·       Increased activity in the open arms will be considered as less anxiety. The maze will be cleaned with 70% ethanol solution after each test to avoid olfactory cues.

 

5.0 END POINT PARAMETER(S):

·       Clinical observation

·       No. of entry and time spent in closed arm

·       No. of entry and time spent in open arm

·       % activity.

 

6.0  REFERENCE(S):

6.1 Dongye He, Xitao Wang, Ping Zhang, Xinxing Luo, Xiaoyu Li, Lili Wang, Shuying Li, and Yongping Xu. Evaluation of the Anxiolytic and Antidepressant Activities of the Aqueous Extract from Camellia euphlebia Merr. ex Sealy in Mice. Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 618409, 8 pages http://dx.doi.org/10.1155/2015/618409

6.2 Long Cheng, Guo-feng Pan, Xiao-bo Sun, Yun-xiang Huang, You-shun Peng, and Lin-yan Zhou. Evaluation of Anxiolytic-Like Effect of Aqueous Extract of Asparagus Stem in Mice. Evidence-Based Complementary and Alternative Medicine Volume 2013, Article ID 587260, 10 pages http://dx.doi.org/10.1155/2013/587260

6.3 Richard G. Lister. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (t 987) 92 : 18~185

6.4 Atul R. Chopade, Prakash M. Somade, Pratik P. Somade, Suraj N. Mali. Identifcation of Anxiolytic Potential of Niranthin: In‑vivo and Computational Investigations. Natural Products and Bioprospecting (2021) 11:223–233.

6.5 B.S. Thippeswamy, Brijesh Mishra, V.P. Veerapur and Gourav Gupta. Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety. Indian J Pharmacol. 2011 Feb; 43(1): 50–55.

                                    END OF DOCUMENT

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1. List of All SOPs and Documents for In-vivo Laboratory

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5.List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

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