1.0 PURPOSE
To design a
Standard Operating Procedure that describes the procedure for operation and maintenance of the CO2 chamber.
2.0
SCOPE
This Standard Operating
Procedure (SOP) applies to the operation, and maintenance of CO2 chamber
Pharmacology studies conducted at animal house facilities.
3.0
RESPONSIBILITY
3.1 The quality assurance
department is responsible for keeping SOP ‘Master Copy’ approved through the Quality
Manager.
3.2
The copy of ‘Control Copy’ of
SOP is being distributed in all user departments and placed near related Equipment/Instruments.
4.0
DISTRIBUTION
4.1
The Quality Assurance
department is responsible for keeping SOP ‘Master Copy’ approved through the Quality Assurance Head.
4.2 The copy of ‘Control Copy’ of
SOP is being distributed in the AHF department and placed near related Equipment/
Instrument as Display Copy.
5.0
DEFINITION(S)
5.1
Definition(s)
5.1.1 The CO2 chamber may be used for
the euthanasia of animals that will comfortably fit in the
chamber.
6.0 ABBREVIATION(S)
6.1 Abbreviations
6.1.1
SOP : Standard Operating Procedure
6.1.2
AHF : Animal House Facility
6.1.3
PPEs : Personal Protective Equipment
7.0
PROCEDURE
7.1
Introduction
The CO2 chamber
may be used for the euthanasia of animals that will comfortably fit in the
chamber. The chamber shall not be precharged prior to placing animals inside.
It is used for animals and is Located in the Necropsy/Surgery room.
7.2
Instrument description
7.2.1 Carbon dioxide gas (CO2)
is heavier than air and will sink to the bottom of the cage. Animals will
quickly lose consciousness and stop breathing when exposed to the gas.
7.2.2 CO2 delivered by a local gas
tank, the investigator must confirm that the tank has enough gas available and,
before opening the gas delivery needle valve, adjust the gas delivery pressure
to between 20 -30 PSI.
7.3
Operations
7.3.1
Check the pressure to ensure
that the cylinder is appropriately charged. The pressure gauge should read
greater than 500 psi. When the pressure falls below 500 psi, notify the manager for
cylinder replacement. To check the pressure in the tank, close the needle valve
on the side of the regulator/flow meter opposite the cylinder. Open the tank
valve. The regulator should read the pressure remaining in the tank. Transfer animals in their home cage with
filter lid to N110E by cart. Use scotch tape to cover the auto water hole if
applicable.
7.3.2 Open the chamber top, place the
animal in the chamber, and set the lid in place. The lid should fit securely in
place over the chamber.
7.3.3 With the needle valve closed
and the tank valve open, adjust the knob at the front of the regulator/flow
meter to a flow that will displace 20% of the volume of the chamber per minute.
For a chamber that is 24in x 18in x 14in, the flow rate should be set at 20Lpm.
(Turning the knob to the left will lower the flow rate and to the right will
raise the rate) With the proper flow selected, open the needle valve to allow
CO2 to flow into the chamber. The animal should be monitored constantly during
this procedure.
7.3.4 Once the animal has lost
consciousness and all movement has stopped, turn the flow of gas to 100% of the
volume of the chamber per minute. For a chamber that is 24in x 18in x 14in, the
flow rate should be set at 100Lpm (if the flow meter does not read up to
100Lpm, turn the flow to the highest level). Allow CO2 to flow at this rate for
at least 1 minute. After no respiration is evident, turn the cylinder off.
7.3.5 Animals should be left in the
closed chamber at least 5 minutes after all movement and respirations have
stopped.
7.3.6 Death should be ensured by
checking for a pulse and/or blink reflex. After no detectable pulse or. After
death has been assured, dispose of the carcass.
7.3.7
If multiple animals are to be
euthanized, the chamber should be left open to allow the CO2 in the chamber to
dissipate before placing the next animal in the chamber. Clean the chamber of
all urine and feces before the next animal is placed in the chamber.
7.3.8 When all euthanasias are
complete, check the cylinder valve to be sure that it is securely closed. Clean
the inside of the chamber thoroughly with a disinfectant/cleaner solution.
Fig 1: CO2 flow rate
7.4
Maintenance
7.4.1
Clean units periodically by
wiping them down with a mild disinfectant and a soft cloth.
7.4.2
Any additional
maintenance/service should be performed by authorized personnel.
8.0
PRECAUTIONS
8.1 All PPEs like an apron, gloves,
mask, head caps, and shoe cover should be worn before using the instrument.
8.2
Press Keys softly.
8.3 Clean the equipment with a dry
piece of robe or a lightly moistened one, do not use organic solutions.
8.4
Never use abrasive products or
dissolvents.
8.5
Never pour water or liquids on
the equipment.
8.6 Training on the use of the
Euthanex chamber is required and will be scheduled through the Technical
Services and Training Coordinator or the Facility Manager prior to personnel
performing CO2 euthanasia. Assistance is available from FLSC staff upon
request.
8.7 Do not euthanize animals in the
animal rooms- animals must be removed to one of the procedure rooms.
8.8 All persons performing
euthanasia must be trained and proficient in the technique(s) employed. The
chamber must be disinfected after use.
9.0
REFERENCES
9.1
Reference
9.1.1
Refer to the manufacturer’s manual for additional information.
END OF THE DOCUMENT
You may like to read these links:
1. List of In-vivo laboratory Vendors (Capex)
2. List of SOPs and Documents for CPCSEA
3. List of All SOPs and Documents for Laboratory Instruments and Equipment
4. List of All SOPs and Documents for Animal House Facility
5. List of All SOPs and Documents for In-vivo Laboratory
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