1. Objective: To lay down the procedure for the operation of Flash chromatography.
2. Responsibility: It is the responsibility of the Technical Assistant, Technical Manager, Quality Manager, and all those individuals who are working on the instrument.
3. Instrument Details:
Make-:............................, Model-:.....................................
4. Procedure:
· Enter into the instrument logbook (in and out time)
4.1 Switch On
· Check the electrical plug points and turn On the switch.
· Check the solvent level and ensure that there is enough solvent for the whole run.
· Start the auto prime for cleaning the lines.
· Start manual prime if you are using a different solvent from the previous user.
· Ensure the lines are kept in the same solvent reservoir as they were programmed (preferably in ethyl acetate & hexane).
· Put all the racks (fraction collector) in place.
· Download the methods if already exists or prepare them according to your TLC.
· Set the detection wavelength according to your compound's UV profile (two-wavelength can be set).
· Mention the amount of solvent which you are going to collect in tubes.
· Ensure there is no solvent leakage.
· Use the column according to your sample quantity (e.g. for the 4 g column you can use a maximum 400 mg of the sample).
· Specify the type of sample loading (solid or liquid).
· For solid loading adsorb your sample on the stationary phase.
· Ensure the surrounding is clutter-free.
4.2. Switch Off
· It is automated flushed and purged, so wait for the required time after the run completes.
· Save your run or take its PDF version on the USB drive.
· Remove the cartridges and column(s).
· Finally switch off the instrument and main switch.
· Signoff in instrument logbook.
5. Unattended Operations of instruments:
· Can be run for a small period of time (~30 mins),
· Overnight operations are not allowed
6. Cleaning:
· Clean the sample preparation area and nearby area of the instrument before logging off
7. Precautions for use:
· Ensure that the column is free from any contamination, by washing with a suitable solvent(s).
· Ensure that solvent reservoirs have a proper lid.
· Ensure that rack has emptied after run over.
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for Laboratory Instruments and Equipment
2. List of All SOPs and Documents for Chemistry Laboratory
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment