1. Objective: To lay down the procedure for the operation of GC-MS/MS.
2. Responsibility: It is the responsibility of the Technical Assistant, Technical Manager, Quality Manager, and all those individuals who are working on the instrument.
3.
Instrument Details:
Make-: .......................... Model-: .....................................................
4. Purpose: This SOP provides an authorized procedure for the operation and maintenance of GC-MS/MS.
5. Scope: This SOP covers the procedure for the operation and maintenance of GC-MS/MS.
6. Principle: GCMSMS is a technique by which a mixture of samples is separated into components using a capillary column meant for gas chromatography coated with a suitable phase and eluting the separated components by employing a suitable gas mobile phase and variation of temperature.
Before you turn on the MS:
· Carrier
gas must be at least 99.999% purity is plumbed to GC with the recommended.
· A capillary column is installed in the GC inlet and in the GC-MS interface.
7.
Procedure:
· Enter
in the log book.
· Switch
on the gases (Helium and Nitrogen) from the cylinder and wall panel. Make sure that
cylinder outlet pressure is between 4 - 6 kg.
· Make
sure the instrument is on an un-uninterrupted power supply.
· Power
on the instrument through GC and MS power buttons.
· Switch
on the PC and launch software from the icon
· Wait
for 5 minutes and then the load conditioning method.
· Keep
the system on conditioning for 4 hrs.
· Load
the method in which you want to analyze the method. Set the ALS, Inlets,
column, oven, Aux heater, configuration, and readiness as desired. The method file
can be saved by assigning a suitable name based on the type of analysis to be
undertaken in the method file for easy retrieval at a later time or date. This
would help in saving time setting the parameters of the system required for
performing the analysis by the click of the method download button. Allow the
system to attain the required parameters for the analysis to be performed.
· In
the instrument control panel, click the MS tune icon to display the GC-QQQ Tune
dialogue box.
· Make
sure "EI hisenseautotune" is selected. Click on Autotune tab. Tuning
will start automatically. Click ok
· Do
Check Tune by clicking on the check tune tab.
· Make
sure each parameter is ok. Click close. Again click on "close" and
then "ok" to go to instrument control.
· For
injecting a single sample, put the sample on desired vial position of the autosampler.
Also,
make
sure the rinsing solvent is filled in wash vials. Click on the arrow of the run method.
· Enter
data path, data file name, sample name, and vial no. Click on "ok and run
method".
· For
running sequence, Click on sequence and Edit sequence:
· Make
desired lines, enter sample name, vial no, method path, Method, Data path, Data
file name, and sample type. Click ok.
· Make
sure the data file name is different in each line otherwise, data will be
overwritten.
· Click
run sequence under the sequence tab.
· Enter the data file directory and click "run".
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for Laboratory Instruments and Equipment
2. List of All SOPs and Documents for Chemistry Laboratory
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