STP FOR DETERMINATION OF FRUCTOSE- GLUCOSE RATIO IN HONEY

1.0 PURPOSE

The purpose of the document is to establish a general and uniform method for the determination of the fructose-glucose ratio in honey.

2.0 SCOPE

This standard testing procedure is suitable for the determination of fructose- glucose ratio in honey.

3.0 RESPONSIBILITY

 

The expert and trained chemist are responsible for following this procedure. The technical manager is responsible for implementing this procedure.

4.0 REQUIREMENTS

4.1 Apparatus: NA

4.2 Glassware

4.1.1 Iodine Flask-250 ml

4.1.2 Burette-50 ml

4.1.3 Pipett-25 ml,50 ml

4.1.4 Volumetric Flask-250 ml

4.3 Reagent

4.2.1 Iodine solution: 0.05 N

4.2.2 Sodium Hydroxide solution: 0.1 N

4.2.3 Conc. Sulphuric Acid

4.2.4 Sodium thiosulphate solution: 0.05 N

5.0 PROCEDURE

5.1 Take 50 ml of the prepared solution for total reducing sugar in a 250 ml Iodine flask with the help of a pipette.

5.2 Add 40 ml Iodine solution(0.05 N) in flask.

5.3 Add 25 ml 0.1N NaOH solution in a flask and place in the dark for 20 min.

5.4 Acidify with 5 ml H2SO4 (conc.)

5.5 Titrate the excess of iodine against standard (0.05N) sodium thiosulphate solution.

5.6 Color disappears and notes down the reading.

5.7 Conduct a blank with 50 ml water in place of the sample solution.

6.0 CALCULATION

7.0 REVISION/CHANGE HISTORY: NA

8.0 ATTACHMENTS

· Appendix-1: FLOW CHART FOR FRUCTOSE- GLUCOSE RATIO

9.0 REFERENCES

· Annex C of IS 4941:1994;RA 2008; Amendment -1, 2011

 

 

                                                                      END OF THE DOCUMENT

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