STANDARD OPERATING PROCEDURE FOOD AND WATER RESTRICTION IN RODENTS
1.0 Purpose
This Standard Operating Procedure (SOP) outlines the procedures for food and water restriction in rodents. Food and water restrictions may be necessary for certain research protocols, but it is important to minimize animal discomfort and ensure that the animals remain healthy.
2.0 Scope
2.0 Scope
This SOP applies to all laboratory personnel who are responsible for the care and feeding of rodents.
Responsibilities The Principal Investigator (PI) is responsible for ensuring that all food and water restriction protocols are scientifically justified and approved by the Institutional Animal Care and Use Committee (IACUC). Laboratory personnel are responsible for following this SOP and implementing food and water restriction protocols as approved by the IACUC.
Responsibilities The Principal Investigator (PI) is responsible for ensuring that all food and water restriction protocols are scientifically justified and approved by the Institutional Animal Care and Use Committee (IACUC). Laboratory personnel are responsible for following this SOP and implementing food and water restriction protocols as approved by the IACUC.
3.0 Procedure
Plan the restriction. Determine the duration of the restriction.
Determine the level of restriction (e.g., 70% of ad libitum intake).
Calculate the amount of food or water to provide each animal.
Obtain approval from the IACUC.
Prepare the animals. Weigh the animals and record their baseline body weights.
Assign the animals to groups based on their body weights.
Start the restriction.
Monitor the animals. Weigh the animals at least twice weekly.
Monitor the animals for signs of dehydration or malnutrition.
Adjust the level of restriction as needed.
End the restriction. Gradually increase the amount of food or water provided to the animals until they are returned to ad libitum intake.
4.0 Records
The following records must be maintained for all food and water restriction protocols: Protocol number
Date of approval by the IACUC
Duration of the restriction
Level of restriction
Amount of food or water provided to each animal
Body weights of the animals at baseline and throughout the restriction
Any signs of dehydration or malnutrition
Any adjustments to the level of restriction
Date the restriction was ended
5.0 Additional Considerations
Date of approval by the IACUC
Duration of the restriction
Level of restriction
Amount of food or water provided to each animal
Body weights of the animals at baseline and throughout the restriction
Any signs of dehydration or malnutrition
Any adjustments to the level of restriction
Date the restriction was ended
5.0 Additional Considerations
Food and water restrictions can be stressful for rodents. It is important to minimize the duration and severity of the restriction as much as possible.
Rodents should have access to food and water for at least 15 minutes per day, even during restriction.
Rodents should be provided with a highly palatable diet during restriction to encourage them to eat.
Rodents should be monitored closely for signs of dehydration or malnutrition.
If an animal shows any signs of distress, the restriction should be terminated immediately.
Rodents should have access to food and water for at least 15 minutes per day, even during restriction.
Rodents should be provided with a highly palatable diet during restriction to encourage them to eat.
Rodents should be monitored closely for signs of dehydration or malnutrition.
If an animal shows any signs of distress, the restriction should be terminated immediately.
6.0 References
This SOP is based on the following guidelines: Guide for the Care and Use of Laboratory Animals (8th edition)
American College of Laboratory Animal Medicine (ACLAM) Policy on Rodent Food and Water Restriction
American College of Laboratory Animal Medicine (ACLAM) Policy on Rodent Food and Water Restriction
END OF THE DOCUMENT
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