SOP for Equipment operating procedure- surgical pathology Automatic tissue processor-Surgical Pathology Automatic Tissue Processor Operation

Equipment operating procedure- surgical pathology Automatic tissue processor-Surgical Pathology Automatic Tissue Processor Operation

1.0 Objective: This Standard Operating Procedure (SOP) outlines the steps and guidelines for the safe and efficient operation of the Surgical Pathology Automatic Tissue Processor in the laboratory setting.

2.0 Equipment:

Surgical Pathology Automatic Tissue Processor

Personal Protective Equipment (PPE) - lab coat, gloves, safety goggles

Specimen containers and labels

Reagents required for tissue processing

Maintenance tools and supplies

3.0 Precautions:

Read and understand the manufacturer's instructions and safety guidelines before operating the tissue processor.

Wear appropriate PPE to protect against chemical exposure and potential splashes.

Ensure the working area is clean and free from clutter.

Avoid contact with reagents and chemicals. Handle them carefully following safety data sheets.

Never put any solvent in paraffin Station

Change Activated Carbon filters at regular intervals depending upon the concentration of reagents and usage.

To avoid overheating of paraffin or wax check the temperature at regular intervals

4.0 Procedure:

4. 1. Preparation:

4.1.1. Verify that the tissue processor is clean and free from any residue from the previous run.

4. 1.2. Check the reagent levels and ensure there's an adequate supply for the planned processing.

4. 1.3. Prepare the tissue samples by appropriately labeling the containers with relevant patient information.

4. 1.4. Power on the tissue processor and allow it to initialize.

4. 2. Loading of Specimens:

4. 2.1. Open the tissue processor's loading chamber following the manufacturer's instructions.

4. 2.2. Place the labeled tissue specimens into the processing baskets or cassettes according to the specified protocol.

4. 2.3. Close the loading chamber securely to prevent any leakage.

4. 3. Reagent Loading:

4.3.1. Refer to the tissue processor manual to identify the correct reagent containers and their placement.

4.3.2. Pour the required reagents into the designated reservoirs following the recommended volumes and sequence.

4.3.3. Ensure that reagent containers are tightly closed to prevent spillage.

4.4. Program Selection:

4. 4.1. Select the appropriate processing program based on the tissue type and desired processing parameters.

4. 4.2. Enter the required processing time, temperature, and agitation settings following the laboratory's established protocols.

4. 5. Initiating the Processing Cycle:

4. 5.1. Start the tissue processing cycle as per the programmed settings, the current order, concentration & run time of reagents is as follows:

10 % Formalin = 2hrs

10% Formalin = 2hrs

20% Alcohol I = 1hrs

95% Alcohol II 1hr

Absolute Alcohol III = 1hr

Absolute Alcohol IV =1hr

Acetone I = 30 minutes

Acetone II = 30 Minutes

Xylene I = 30 minutes

Xylene II = 30 Minutes

Wax I = 2 hrs

Wax II = 2 hrs

4. 5.2. Monitor the processor periodically to ensure proper functioning and address any issues promptly.

4. 5.3. Avoid interrupting the cycle unless necessary to prevent compromising the tissue processing.

4. 6. Completion of Processing:

4. 6.1. Once the processing cycle completes, carefully open the processor and remove the processed tissue specimens.

4. 6.2. Dispose of the used reagents following laboratory waste disposal protocols.

4. 6.3. Clean the tissue processor according to the manufacturer's instructions and laboratory guidelines.

4. 7. Maintenance:

4. 7.1. Regularly schedule and perform maintenance tasks as recommended by the manufacturer.

4. 7.2. Keep a log of maintenance activities, including dates, tasks performed, and any issues encountered.

4. 8. Shutdown:

4. 8.1. Power off the tissue processor after use.

4. 8.2. Clean the exterior surfaces and ensure the area is left tidy for the next user.

4. 9. Documentation:

4. 9.1. Record details of tissue processing activities, including patient information, processing parameters, and any deviations encountered.

4. 9.2. Maintain records of equipment maintenance and calibration.

4. 10. Safety Measures:

4. 10.1. Handle chemicals and reagents with caution, following proper safety protocols and disposal procedures.

4. 10.2. Report any malfunctions or safety concerns regarding the tissue processor immediately to the supervisor or maintenance personnel.

4. 11. Training:

4. 11.1. Ensure that all personnel operating the tissue processor are adequately trained and competent in using the equipment safely and effectively.

This SOP should be reviewed periodically and updated as needed to reflect any changes in equipment operation, safety guidelines, or laboratory protocols.

END OF THE DOCUMENT

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