Applications of Rotary Evaporator with Chiller in Natural Product Studies
A Rotary Evaporator
(Rotavap) with Chiller is an essential piece of laboratory
equipment used for the efficient and gentle removal of solvents
from samples, especially in natural product research
where heat-sensitive bioactive compounds are commonly present.
Key Applications
in Natural Product Studies:
1. Solvent
Removal:
o
Evaporation of extraction solvents (e.g.,
ethanol, methanol, hexane) from plant or herbal extracts.
o
Concentrating crude extracts before further
purification.
2. Sample
Concentration:
o
Reducing sample volume to concentrate active
constituents.
o
Preparing samples for HPTLC, HPLC, GC-MS, NMR,
or bioassays.
3. Solvent
Recovery:
o
Reclaiming expensive or environmentally
sensitive solvents for reuse.
4. Purification
Steps:
o
Intermediate concentration step between
liquid-liquid extraction and chromatographic purification (e.g., column
chromatography).
5. Drying
of Fractions:
o
Concentrating chromatography fractions for
subsequent analysis or bioactivity testing.
6. Temperature-Sensitive
Compounds:
o
Gentle evaporation under reduced pressure
prevents degradation of heat-labile natural products like alkaloids,
flavonoids, and essential oils.
Specifications
Needed for Natural Product Studies
To use a rotary evaporator effectively for natural product work, consider
the following specifications:
Rotary Evaporator Specifications:
Chiller/Circulator Specifications:
Technical
specifications for vertical rotary vacuum evaporator, vacuum pump and chiller:
Rotary vacuum evaporator with motorised auto jack-up
function during power failure (with digital water bath)
· Driving Unit: Encased
induction motor with electronic speed control ·
Condenser: Vertical Double spiral glass coil
· Drive Unit: Motorized drive unit
· Jack up Function: Electronic
quick action jack and automatic lifting of the flasks during a power failure
· Rotation Speed: up to 280 rpm or
better with microprocessor control.
· Display : digital display of
rotation speed and heating bath.
· Speed display : Digital
· Sample Flask : 1L, Pear Style
· Receiving flask : 1L, round
style with ball joint.
· Evaporating flask from 50-4000
ml can be used on the same joint adapter without additional connections.
· 1 liter Evaporating Flask and
Receiving Flasks should be provided in standard scope of supply.
· Vacuum seal : PTFE seal (seal
should also be covered in standard warranty period of three year)
· Liquid Contact parts : Glass
& PTFE
· Includes with Digital Water Bath
o Temp.
control range: Ambient temp. to 210° C or higher.
o
Accuracy
: ± 1°C o Bath features : Inner
Teflon coating and outer heat resistant cover
o
Bath
capacity : should be between 4 to 5L
o
Temp
controller: Microprocessor based PID Control
o
Safety
features: Overheating protector, auto cut off at 5˚C, secondary over
temperature cut off 250˚C
A.
VACUUM
PUMP:
· Two-stage oil-free
diaphragm pump made from chemically resistant material.
· Suction capacity in m3 /hour :
2.0 m3 /h or better.
· Vacuum pump should have ultimate
vacuum of 7mbar or better.
· Power input in watt : 180
· Supply voltage (V/Hz): 230/50.
B.
CHILLER
· Temperature range: -10˚C to
ambient
· Precise temperature control of ±
0.2˚C
· Flow: more than 6l/min
· Cooling capacity up to 560 watts
@20˚C
· Fluid level indicator.
· Low flow shut off and high/low
temperature alarms.
· Digital display.
Importance about Rotary Evaporator
A Rotary Evaporator with Chiller is vital for:
·
Gentle and efficient solvent removal,
·
Concentration of plant extracts, and
·
Preserving the integrity of
temperature-sensitive phytochemicals.
Choosing the right specifications (especially in terms of vacuum
control, temperature range, and cooling capacity) ensures
reproducibility and safety in natural product
isolation and characterization workflows.
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for Laboratory Instruments and Equipment
2. List of All SOPs and Documents for Chemistry Laboratory
4. SOP for Rotary Evaporator (Rotavapor).
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