SOP FOR WATER BATH | STANDARD OPERATING PROCEDURE FOR WATER BATH

1.0 OBJECTIVE

1.1 To lay down a standard procedure to be followed for operation and calibration of Water Bath in Animal House Facility.

2.0 SCOPE

2.1 This Standard Operating Procedure (SOP) shall be applicable for operation and calibration of Water Bath in Animal House Facility.

3.0 RESPONSIBILITY

3.1 Head / Designee

3.2 All Personnel operating Water Bath in Animal House Facility.

4.0 DEFINITIONS

4.1 Nil

5.0 PROCEDURE

5.1 Operating Procedure

5.1.1 If the water bath is not set up already, do a fit for use inspection and remove any materials that are in the water bath.

5.1.2 Add an appropriate amount of water (preferably distilled).

5.1.3 Plug in the chord of the instrument to the power source and switch on the MAINS.

5.1.4 Set the water bath temperature controller knob to desired temperature and let the temperature reach pre-set value.

5.1.5 Confirm the temperature by using a calibrated thermometer.

5.1.6 If the water bath is already set up and lower temperature is required for next use the bath can be cooled by adding cold water.

5.1.7 Label the water bath with an “IN PROCESS” label.

5.1.8 Remove all the water after use and dry the instrument before covering the lid.

Notes:

· When filling the water bath use only distilled water.

· Never place metal tube racks or clamps into the water bath that can get corroded.

· Adjust the water level carefully since vessels that is to be added to the water bath will displace the water, increasing the apparent volume of the water bath.

· Make sure that the tubes or vessels will not fall over and lose its content/s in the water bath.

· Tape, pen, and marker tend to come off from labels in the water bath. Make sure that you are using tape, or markers that are water-resistant.

· Set temperature and rheostats can be accidentally changed.

· Make sure that you use an independent thermometer to check the temperature of the water bath.

5.2 Calibration

4.2.1 The water bath calibration shall be done internally with the help of calibrated thermometer.

4.2.2 Calibration shall be done once in every 6 months or when required (after any major maintenance work).

4.2.3 Compare the results with the permitted variation (Acceptance criteria).

4.2.4 Maintain the calibration record as per format No. F…………………..

4.2.5 If the instrument fails in calibration call the authorized service engineer.

6.0 ENCLOSURES

6.1 Format

Sr. No.	Format Title	Format No	No. of Pages
1.	Calibration Record of Water Bath Temperature	F………………….	01

7.0 ABBREVIATIONS

7.1 SOP : Standard Operating Procedure

7.2 QAD : Quality Assurance Department

7.3 AHF : Animal House Facility

8.0 REFERENCES

8.1 Users Manual

8.2 In-house

9.0 REVISION HISTORY

END OF DOCUMENT

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