2.0 RESPONSIBILITY: The Research staff is responsible for assigning all rodent species to unique
intramural identification number during the receiving of the animal.
3.0 MATERIALS:
3.1 Notching equipment
3.2 Ear punch
3.3 Permanent Marker
4.0 PROCEDURE:
4.1 Ear Notching/Punching (Rat and mice):
3.0 MATERIALS:
3.1 Notching equipment
3.2 Ear punch
3.3 Permanent Marker
4.0 PROCEDURE:
4.1 Ear Notching/Punching (Rat and mice):
4.1.1 This method should not be used in mice under 2 weeks of age.
4.1.2 The mouse is restrained by the scruff and ear punch is used to create holes and or notches in the ears, following an identification chart.
4.1.3 Whenever possible, a simple code is used to limit the number of notches/punches.
4.1.4 The identification chart should be readily available in the animal room to allow prompt identification of individuals.
4.2 Tattooing (Rabbit):
4.2.1 A permanent marker is used to write the numbers on the inner side of ear.
4.2.2 This procedure does not require anesthesia.
4.2.3 The animal identification number should be given according to the month, year of purchase and number of animal. Example: Animal purchased in June 2012 and number of animals is 50 than identification number would be 61250.
5.0 PRECAUTIONS:
5.1 The animals are handled carefully.
5.2 The animal identification number is put carefully.
5.3 The animals should be checked carefully at the time of receiving of the animals.
6.0 REFERENCES:
6.1 Guide for the Care and Use of Laboratory Animals. National Research Council; National Academy Press, Washington, DC, 1996.
6.2 2003 Supplement on Refinement and Reduction in Production of Genetically Modified Mice. Laboratory Animals, vol. 37, no. 3, Suppl.1 July 2003.
6.3 Cornell Center for Animal Resources and Education, SOP 552, Identification of Mice.
6.4 Balaban RS, Hampshire VA. Challenges in small animal noninvasive imaging. ILARJILAR J. 2001;42:248–262. [PubMed]
6.5 Barahona H, Melendez LV, Hunt RD, Forbes M, Fraser CEO, Daniel MD. Experimental horizontal transmission of herpesvirus saimiri from squirrel monkeys to an owl monkey. J Infect Dis. 1975;132:694–697.[PubMed]
4.1.2 The mouse is restrained by the scruff and ear punch is used to create holes and or notches in the ears, following an identification chart.
4.1.3 Whenever possible, a simple code is used to limit the number of notches/punches.
4.1.4 The identification chart should be readily available in the animal room to allow prompt identification of individuals.
4.2 Tattooing (Rabbit):
4.2.1 A permanent marker is used to write the numbers on the inner side of ear.
4.2.2 This procedure does not require anesthesia.
4.2.3 The animal identification number should be given according to the month, year of purchase and number of animal. Example: Animal purchased in June 2012 and number of animals is 50 than identification number would be 61250.
5.0 PRECAUTIONS:
5.1 The animals are handled carefully.
5.2 The animal identification number is put carefully.
5.3 The animals should be checked carefully at the time of receiving of the animals.
6.0 REFERENCES:
6.1 Guide for the Care and Use of Laboratory Animals. National Research Council; National Academy Press, Washington, DC, 1996.
6.2 2003 Supplement on Refinement and Reduction in Production of Genetically Modified Mice. Laboratory Animals, vol. 37, no. 3, Suppl.1 July 2003.
6.3 Cornell Center for Animal Resources and Education, SOP 552, Identification of Mice.
6.4 Balaban RS, Hampshire VA. Challenges in small animal noninvasive imaging. ILARJILAR J. 2001;42:248–262. [PubMed]
6.5 Barahona H, Melendez LV, Hunt RD, Forbes M, Fraser CEO, Daniel MD. Experimental horizontal transmission of herpesvirus saimiri from squirrel monkeys to an owl monkey. J Infect Dis. 1975;132:694–697.[PubMed]
END OF THE DOCUMENT
You may like to read these links:
1. List of In-vivo laboratory Vendors (Capex)
2. List of SOPs and Documents for CPCSEA
3. List of All SOPs and Documents for Laboratory Instruments and Equipment
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment