SOP FOR LAMINAR AIR FLOW | STANDARD OPERATING PROCEDURE FOR LAMINAR AIR FLOW

 

1.0 PURPOSE

To design the standard operating procedure for operation of Laminar Air Flow.

2.0 SCOPE

2.1 This SOP shall be applicable for the operation of Laminar Air Flow.

3.0 RESPONSIBILITY

3.1 All the users including Scientist(s) and Research associate(s) of Microbiology biology department are responsible for implementation of this SOP and maintaining the Laminar Air Flow.

 

4.0 DISTRIBUTION

4.1 The Quality Assurance department responsible to keep SOP ‘Master Copy’ approved through Quality Manager.

4.2 The copy of ‘Control Copy’ of this SOP is being distributed in  Microbiology department and placed near related Equipment/ Instrument as ‘Display Copy’.

5.0 ABBREVIATIONS and DEFINITIONS

5.1 Abbreviations

5.1.1 SOP : Standard Operating Procedure

5.1.2 UV : Ultra Violet

5.2 Definitions

5.2.1 A laminar flow cabinet or laminar flow closet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials.

6.0 PROCEDURE

6.1 Close the sash of laminar & keep the UV ON for 20 min. Lift the sash to the recommended height.

6.2 Turn on the blower 10 minutes before beginning work to allow adequate air filtration.

6.3 Decontaminate the cabinet surface and surface-decontaminate all materials to be placed inside using 70% ethanol.

6.4 When work is completed, all equipment and supplies from the laminar should be decontaminated and removed from the cabinet.

6.5 The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet.

6.6 Wash hands and any exposed surfaces thoroughly after the clean-up procedure.

7.0 PRECAUTIONS

7.1 Keep the work area of the laminar free of unnecessary equipment or supplies. Clutter inside the laminar may impede proper airflow and the level of protection provided.

7.2 Wear eye protection, a lab coat, closed-toe shoes and disposable gloves to protect yourself as well as your samples from contamination.

7.3 Always use mechanical pipetting aids. Mouth pipetting is not allowed. UV lamps must be turned off when the room is occupied to protect eyes and skin from UV exposure, which can burn the cornea and cause skin cancer.

7.4 Bulky items, such as waste containers and suction collection flasks, should be placed to one side of the interior of the cabinet.

8.0 REFERENCE(S) AND FORMAT(S)

8.1 Reference

8.1.1 User manual.

8.2 Format

8.2.1 Format I- Document History sheet.

8.2.1 Format II- Format for Log Book.

9.0 REVISION HISTORY

9.1 Maintain the history of SOP as per format given in SOP on SOP



                                                         END OF THE DOCUMENT

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