1.0 PURPOSE
To design the standard operating procedure for operation of BioSafety Cabinet.
2.0 SCOPE
2.1 The scope of this SOP applies to BioSafety Cabinet (Class-II) to provide microbe-free work environment necessary for RNA work and other related works.
3.0 RESPONSIBILITY
3.1 All the users including Scientist(s) and Research associate(s) of invitro department are responsible for implementation of this SOP and maintaining the BioSafety Cabinet.
4.0 DISTRIBUTION
4.1 The Quality Assurance department responsible to keep SOP ‘Master Copy’ approved through Quality Manager.
4.2 The copy of ‘Control Copy’ of this SOP is being distributed in invitro department and placed near related Equipment/ Instrument as ‘Display Copy’.
5.0 ABBREVIATIONS
5.1 Abbreviations
5.1.1 SOP : Standard Operating Procedure
5.1.2 BSC : Biosafety Cabinet
5.1.3 UV : Ultra Violet
5.1.4 HEPA : High Efficiency Particulate Absorber
6.0 DEFINITIONS
6.1 Definitions
6.1.1 A biological safety cabinet (Class-II) is a partially enclosed workspace that has built in protection for the worker, the environment, and the material inside of it. Class II biological safety cabinets have open fronts, a vertical laminar air flow, so outside air is run through a filter before it gets inside, and a HEPA (high-efficiency particulate absorber) filter for filtering air from inside before it goes outside the cabinet. It is used in microbiology labs, pharmaceutical labs, and cancer research labs, class II biological safety cabinets protect the bacteria, viruses, and carcinogens being manipulated inside the cabinet while shielding them from outside contamination.
7.0 PROCEDURE
7.1 Close the sash of BSC (Biosafety Cabinet) and keep it the ON for 20 minutes.
7.2 Lift the sash to the recommended height.
7.3 Turn on the BSC blower 10 minutes before beginning work to allow adequate air filtration.
7.4 Decontaminate the cabinet surface and surface-decontaminate all materials to be placed inside the BSC using 70% ethanol.
7.5 When work is completed, all equipment and supplies from the BSC should be decontaminated and removed from the cabinet.
7.6 The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet.
7.7 Wash hands and any exposed surfaces thoroughly after the clean-up procedure.
8.0 PRECAUTIONS
8.1 Bulky items, such as waste containers and suction collection flasks, should be placed to one side of the interior of the cabinet.
8.2 Keep the work area of the BSC free of unnecessary equipment or supplies. Clutter inside the BSC may impede proper airflow and the level of protection provided.
8.3 Wear eye protection, a lab coat, closed-toe shoes and disposable gloves to protect yourself as well as your samples from contamination.
8.4 Always use mechanical pipetting aids. Mouth pipetting is not allowed.
8.5 Heat sources such as Bunsen burners are strictly prohibited inside the BSCs as they significantly disrupt the laminar flow of air.
8.6 UV lamps must be turned off when the room is occupied to protect eyes and skin from UV exposure, which can burn the cornea and cause skin cancer.
9.0 REFERENCES
9.1 Reference
9.1.1 User manual.
END OF THE DOCUMENT
You may like to read theses links:
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4. List of All SOPs and Documents for Clinical Research
5.List of All SOPs and Documents for Laboratory Instruments and Equipments
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